Abstract
The objective of this work was to determine the shifts in the PCR-DGGE profiles of bacterial communities associated with the rhizosphere soil of ginseng at varying age levels. Differences in the dominance of intense DNA bands in the DGGE profile was observed over the age of the plants indicating the fluctuation in the microbial community structure. The bacterial orders of actinomycetales of Actinobacteria and Spingomonadales and Rhizobiales of α-Proteobacteria were predominant in the ginseng soil.
References
Ercolini D (2004) PCR-DGGE fingerprinting: novel strategies for detection of microbes in food. J Microbiol Methods 56:297–314
Yu WJ, Lee BJ, Nam SY, Yang DC, Yun YW (2003) Modulating effects of Korean ginseng saponins on ovarian function immature rats. Biol Pharm Bull 26:2574–2580
Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700
Griffiths BS, Ritz K, Ebblewhite N, Dobson G (1999) Soil microbial community structure: effects of substrate loading rates. Soil Biol Biochem 31:145–153
EPdeB Ferreira, Dusi AN, Xavier GR, Rumjanek NG (2008) Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles. Pesq agropec bras Brasília 43:605–612
Kim MS, Ahn JH, Jung MK, Yu JH, Joo DH, Kim MC, Shin HC, Kim TS, Ryu TH, Kweon SJ, Kim TS, Kim DH, Ka JO (2005) Molecular and cultivation-based characterization of bacterial community structure in rice field soil. J Microbiol Biotechnol 15:1087–1093
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Vendan, R.T., Lee, S.H., Yu, Y.J. et al. Analysis of Bacterial Community in the Ginseng Soil Using Denaturing Gradient Gel Electrophoresis (DGGE). Indian J Microbiol 52, 286–288 (2012). https://doi.org/10.1007/s12088-011-0193-3
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DOI: https://doi.org/10.1007/s12088-011-0193-3