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Biotransformation of α-Pinene to Terpineol by Resting Cell Suspension of Absidia corulea

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Abstract

Microbial biotransformation of monoterpenes results in the formation of many valuable compounds. Many microorganisms can be used to carry out extremely specific conversions using substrates of low commercial value. Absidia corulea MTCC 1335 was examined for its ability to transform α-Pinene enantiomers. The substrates (−)-α-Pinene and (+)-α-Pinene converted to α-terpineol and isoterpineol, were detected in gas chromatographic analysis. The Biotransformation kinetics of the oxidized products were analysed using GC–MS. With both the substrates the products formed were similar and not much difference in the rate of transformation was observed, suggesting no enantioselectivity of organism towards the substrate.

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References

  1. Trudgill PW (1994) Microbial metabolism and transformation of selected monoterpenes. In: Ratledge C (ed) Biochemistry of microbial degradation. Kluwer, Dordrecht, pp 33–61

    Chapter  Google Scholar 

  2. Onken J, Berger RG (1999) Effects of R-(+)-limonene on submerged cultures of the terpene transforming basidiomycete Pleurotus sapidus. J Biotechnol 69:163–168

    Article  PubMed  CAS  Google Scholar 

  3. Abraham BG, Berger RG (1994) Higher fungi for generating aroma components through novel biotechnologies. J Agric Food Chem 42:2344–2348

    Article  CAS  Google Scholar 

  4. Hagedorn S, Kaphammer B (1994) Microbial catalysis in the generation of flavour and fragrance chemicals. Annu Rev Microbiol 48:773–800

    Article  PubMed  CAS  Google Scholar 

  5. Van Rensburg E, Moleleki N, van der Walt JP, Botes PJ, van Dyk MS (1997) Biotransformation of (+)limonene and (–)piperitone by yeasts and yeast-like fungi. Biotechnol Lett 19:779–782

    Article  Google Scholar 

  6. Adams, Demyttenaere JCR, De Kimpe N (2003) Biotransformation of (R)-(+)- and (S)-(−)-limonene to α-terpineol by Penicillium digitatum—investigation of the culture conditions. Food Chem 80:525–534

    Article  CAS  Google Scholar 

  7. Tan Q, Day DF (1998) Bioconversion of limonene to α-terpineol by immobilized Penicillium digitatum. Appl Microbiol Biotechnol 49:96–101

    Article  CAS  Google Scholar 

  8. Demyttenaere JCR, Van Belleghem K, De Kimpe N (2001) Biotransformation of (R)-(+)- and (S)-(−)-limonene by fungi and the use of solid phase microextraction for screening. Phytochemistry 57:199–208

    Article  PubMed  CAS  Google Scholar 

  9. Brzezowska E, Dmochowska Gladysz J, Kolek T (1996) Biotransformation XXXIX. Metabolism of testosterone, androstenedione, progesterone and testosterone derivatives in Absidia coerulea culture. J Steroid Biochem Mol 57:357–362

    Article  CAS  Google Scholar 

  10. Sun DA, Sauriol F, Mamer O, Zamir Lolita O (2001) Reanalysis of the biotransformation of 4(20), 11(12)-taxadiene derivatives. Can J Chem 79(9):1381–1393

    Article  CAS  Google Scholar 

  11. Yang Lin, Dai Jungui (2008) Biotransformation of tetrahydro-alpha-santonins by Absidia coerulea. Nat Prod Res 22(6):499–506

    Article  PubMed  CAS  Google Scholar 

  12. Toniazzo G, Oliveira D, Dariva C, Oestreicher EG, Antunes OAC (2005) Biotransformation of (1S)-(−)alpha-pinene, (−)beta-pinene and (+)limonene by Aspergillus niger ATCC 9642. Biotechnol Appl Biochem 123:837–844

    Article  Google Scholar 

  13. Rozenbaum HF, Patitucci ML, Antunes OAC, Pereira N Jr (2006) Production of aromas and fragrances through microbial oxidation of monoterpenes. Braz J Chem Eng 23(3):273–279

    Article  CAS  Google Scholar 

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Acknowledgments

Authors are thankful to the Director, Indian Institute of Chemical Technology, Hyderabad for providing necessary facilities and encouragement for carrying out of this work.

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Correspondence to U. S. N. Murty.

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Siddhardha, B., Vijay Kumar, M., Murty, U.S.N. et al. Biotransformation of α-Pinene to Terpineol by Resting Cell Suspension of Absidia corulea . Indian J Microbiol 52, 292–294 (2012). https://doi.org/10.1007/s12088-011-0155-9

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  • DOI: https://doi.org/10.1007/s12088-011-0155-9

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