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Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Indian Journal of Microbiology volume 51, pages 182–187 (2011)Cite this article

Abstract

A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E. casseliflavus (4), E. durans (4), E. faecalis (4), E. mundtii (3) and E. avium (2). Of the 57 isolates 40 of them inhibited the growth of at least one indicator bacterium. Based on our PCR results 54 strains possesed enterocin genes. The genes of entA and entB were the most frequently detected structural genes among the PCR positive strains (54 and 53 strains, respectively) and the entB gene was always associated with entA gene. The highest combination of enterocin genes (24 of 54 strains) detected was entA, entB, entP and entL50A/B. The enterocins AS-48 and CylLLS genes were not found. Three enterococcal isolates, 2 E. faecium and 1 E. hirae were not harbour any of tested enterocin genes. No correlation between the presence of enterocin structural genes and the origin of the strain was detected, also no relationship seemed to exist between the tested enterocin genes and the activity spectra of isolates. Genes encoding bacteriocins are widely disseminated among enterocci from different origin and more studies should be done for evaluate industrial potential of bacteriocins.

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Authors and Affiliations

  1. Faculty of Arts and Science, Department of Biology, Adnan Menderes University, 09100, Aydın, Turkey

    Gamze Başbülbül Özdemir, Hacı Halil Bıyık & Melihcan Özteber

  2. ADU BILTEM Epidemiology Unit, Adnan Menderes University, 09100, Aydın, Turkey

    Erman Oryaşın & Bülent Bozdoğan

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  1. Gamze Başbülbül Özdemir
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  2. Erman Oryaşın
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  3. Hacı Halil Bıyık
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  4. Melihcan Özteber
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  5. Bülent Bozdoğan
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Correspondence to Gamze Başbülbül Özdemir.

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Özdemir, G.B., Oryaşın, E., Bıyık, H.H. et al. Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources. Indian J Microbiol 51, 182–187 (2011). https://doi.org/10.1007/s12088-011-0143-0

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Keywords

  • Bacteriocin
  • Enterococcus
  • Enterocin Structural Genes
  • PCR