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Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

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Abstract

A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen for the transformation experiments. Plasmid pBT was used as transformation vector containing the Aspergillus nidulans nitrate reductase gene. A frequency of approximately 3 transformants/μg DNA was obtained using the circular vector pBT. The establishment of a transformation system for V. lecanii is fundamental for genetic manipulation of this microorganism.

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Acknowledgment

The authors would like to express their thanks to Prof. Mrs. Karuna Verma Head, Department of Biological Sciences for her kind support and providing lab facilities.

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Authors and Affiliations

  1. Fungal Biotechnology and Invertebrate Pathology Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, India

    Saba Hasan, Rana Inder Singh & Sandhu Sardul Singh

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  1. Saba Hasan
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  2. Rana Inder Singh
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  3. Sandhu Sardul Singh
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Correspondence to Saba Hasan.

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Hasan, S., Singh, R.I. & Singh, S.S. Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans . Indian J Microbiol 51, 390–395 (2011). https://doi.org/10.1007/s12088-011-0106-5

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  • DOI: https://doi.org/10.1007/s12088-011-0106-5

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