Skip to main content

Advertisement

Log in

RETRACTED ARTICLE: Ecological Significance of Microdiversity: Coexistence Among Casing Soil Bacterial Strains Through Allocation of Nutritional Resource

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

This article was retracted on 21 March 2019

This article has been updated

Abstract

A combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16S rRNA gene sequences can represent distinct eco- and genotypes. A set of eight bacterial strains wherein three were Pseudomonas putida and rest were Acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. These strains had identical 16S rRNA gene sequences and represented the dominant phylotype in the plateable fraction. Each strain utilized a specific combination of 154 carbon substrates, and the niche overlap indices were low, suggesting that each strain occupied a different ecological niche. Our results have implications for assessment of the diversity and biogeography of bacteria and increase the perception of natural diversity beyond the level of 16S rRNA gene sequences. It is worthwhile approach to explore prokaryotic diversity in different ecological niches.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

Change history

  • 21 March 2019

    The Editor-in-Chief has retracted this article [1] because of significant overlap with a previously published article by Jaspers and Overmann [2]. All authors agree to this retraction.

  • 21 March 2019

    The Editor-in-Chief has retracted this article [1] because of significant overlap with a previously published article by Jaspers and Overmann [2]. All authors agree to this retraction.

References

  1. Brock T (1987) The study of microorganisms in situ: progress and problems. In: Gray TRG, Jones JG (eds) Ecology of microbial communities (Fletcher M. Cambridge University Press, Cambridge

    Google Scholar 

  2. Wilson EO (1994) The diversity of life. Penguin, London

  3. Finlay BJ (2002) Global dispersal of free living microbial eukaryote species. Science 296:1061–1063

    Article  CAS  Google Scholar 

  4. Curtis TP, Sloan WT, Scanneu J (2002) Estimating prokaryotic diversity its limits. Proc Natl Acad Sci USA 99:10494–10499

    Article  CAS  Google Scholar 

  5. Curtis TP, Sloan WT (2004) Prokaryotic diversity its limits: microbial community structure in nature implications for microbial ecology. Curr Opin Microbiol 7:221–226

    Article  Google Scholar 

  6. Amann R, Ludurg W, Schleifer KH (1995) Phylogentic indentification insitu detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Pernthaler J, Glockner FO, Unterholzner S, Altreider A, Psenner R, Amann R (1998) Seasonal community population dynamics of pelagic bacteria and archaea in a high mountain lake. Appl Environ Microbiol 60:4299–4306

    Google Scholar 

  8. Ward DM, Ferris MJ, Nold SC, Bateson MM (1998) A natural view of microbial biodiversity within not spring cyanobacterial mat communities. Microbial Mol Biol Rev 62:1353–1370

    CAS  Google Scholar 

  9. Moore LR, Rocap G, Chisholm SW (1998) Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes. Nature 393:464–467

    Article  CAS  Google Scholar 

  10. Postius C, Ernst A (1999) Mechanism of dominance: coexistence of picocyanobacterial genotypes in a freshwater ecosystem. Arch Microbiol 172:69–75

    Article  CAS  Google Scholar 

  11. Gray ND, Head IN (2001) Linking genetic identities function in communities of uncultured bacteria. Environ Microbiol 3:481–492

    Article  CAS  Google Scholar 

  12. Böddinghaus B, Wolters J, Heikens W, Bottger EC (1990) Phyhogenetic analysis and identification of deferent serovars of Mycobacterium intracellulare at the molecular level. FEMS Microbiol Lett 70:197–204

    Article  Google Scholar 

  13. Lebuhn M, Achourak W, Schloter M, Berge O, Meier H, Hartmann A, Heulin T (2000) Taxonomic characterization of Ochrobactrum sp. isolated from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignunense sp. nov. Int J Syst Evol Microbiol 50:2207–2223

    Article  CAS  Google Scholar 

  14. Trebesius K, Harmsen D, Rakin A, Schmelz J, Heesemann J (1998) Development of RNA targeted PCR in situ hybridization with fluorescently labeled oligonucleotides for detection of Yersinia species. J Clin Microbiol 36:2557–2564

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Ash C, Farrow JAE, Dorsch M, Stackebrandt E, Collins MD (1991) Comparative analysis of B. anthracis, B. cereus and related species on the basis of reverse transcriptase sequencing 16S rRNA. Int J Syst Bacteriol 41:343–346

    Article  CAS  Google Scholar 

  16. Read TD, Peterson SN, Tourasse N, Baillie LW, Paulsen IT, Nelson KE, Tettelin H, Fouts DE, Eisen JIA, Gill SR, Holtzapple EK, Okstad OA, Helgason E, Rilstone J, Wu M, Kolenay JF, Beanan MJ, Dedcon RJ, Brinkac LM, Gurinn M, Deboy RT, Madpu R, Daugherty SC, Durkin AS, Haft DH, Nelson WC, Peterson JD, Pep M, Khouri HM, Radune D, Benton JL, Mahamoud Y, Jiang L, Hance IR, Weidman JF, Berry KJ, Plant RD, Wolf AM, Watkins KL, Nierman WC, Hazen A, Cline R, Redmond C, Thwaite JE, White O, Salzberg SL, Thomason B, Friedlander AM, Kuchler TM, Hanna PC, Kslsto AB, Fraser CM (2003) The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423:81–86

    Article  CAS  Google Scholar 

  17. King EO, Ward MK, Raney DE (1954) Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307

    CAS  PubMed  Google Scholar 

  18. Bazzicalupo M, Fani R (1994) The use of RAPD for generating specific DNA probes for microorganisms. In: Clapp J (ed) Methods in molecular biology. Humana Press Inc, Totowa, pp 155–175

    Google Scholar 

  19. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    Article  CAS  Google Scholar 

  20. Wilson M, Lindow SE (1994) Coexistence among epiphytic bacterial populations mediated through nutritional resource partitioning. Appl Environ Microbiol 60:4468–4477

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Rohlf FJ (1993) NTSYSpc numerical taxonomy and multivariate analysis system for the IBM PC microcomputer (and compatibles). Applied Biostatistics, Setauket, NY

    Google Scholar 

  22. Bachner BR, Savagean MA (1977) Generalized indicator plate for genetic, metabolic and taxonomic studies with microorganisms. Appl Environ Microbiol 33:434–444

    Google Scholar 

  23. Kirk JL, Beaudette LA, Hart M, Moutoglis P, Klironomos JN, Lee H, Trevors JT (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58:169–188

    Article  CAS  Google Scholar 

  24. Rossellö-Mora R, Amann R (2001) The species concept for prokaryates. FEMS Microbiol Rev 25:39–67

    Article  Google Scholar 

  25. Dykhuizen DE (1998) Santa Rosalia revisited: why are there so many species of bacteria? Antonie Van Leeuwen 73:25–33

    Article  CAS  Google Scholar 

  26. Jaspers E, Overmann J (2004) Ecological significance of microdiversity: identical 16S rRNA gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies. Appl Environ Microbiol 70:4831–4839

    Article  CAS  Google Scholar 

  27. Perna NT, Plunkett G III, Burland V, Man B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, Posfai G, Hackett J, Klink S, Boutin A, Shao Y, Miller L, Grotbeck EJ, Danis NW, Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Anantharaman TS, Lin J, Yen G, Schwartz DC, Welch RA, Blattner FR (2001) Genome sequence of enterohaemorrhagic E.coli 0157: 117. Nature 409:529–533

    Article  Google Scholar 

  28. Sass H, Wieringa E, Cypionka H, Babenzien HD, Overmann J (1998) High genetic physiological diversity of sulfate reducing bacteria isolated from oligotrophic lake sediment. Arch Microbiol 170:243–251

    Article  CAS  Google Scholar 

  29. Sikorski J, Nohle M, Wackernagel W (2002) Identification of complex composition, strong strain diversity directional selection in local pseudomonas stutzeri populations from marine sediment and sials. Environ Microbiol 4:465–476

    Article  CAS  Google Scholar 

  30. Wieringa EJ, Overmann M, Cypionka H (2000) Detection of abundant sulphate reducing bacteria in marine oxic sediment layers by a combined cultivation and molecular approach. Environ Microbiol 2:417–427

    Article  CAS  Google Scholar 

  31. Beja O, Koonin EV, Aravind L, Taylor LT, Seitz H, Stein JL, Bensen DC, Feldman RA, Swanson RV, Dehong EF (2002) Comparative genomic analysis of archeal genotypic variants in a single population in two different oceanic provinces. Appl Environ Microbial 68:335–345

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was performed at Department of Biotechnology, BU, Bhopal with kind cooperation of Dr. Anil Prakash, Head, Department of Biotechnology & Bioinformatics Centre, Bhopal.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Devendra Kumar Choudhary.

Additional information

The Editor-in-Chief has retracted this article because of significant overlap with a previously published article by Jaspers and Overmann. All authors agree to this retraction.

About this article

Cite this article

Choudhary, D.K., Johri, B.N. RETRACTED ARTICLE: Ecological Significance of Microdiversity: Coexistence Among Casing Soil Bacterial Strains Through Allocation of Nutritional Resource. Indian J Microbiol 51, 8–13 (2011). https://doi.org/10.1007/s12088-011-0068-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-011-0068-7

Keywords