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Contribution of Catalase and Superoxide Dismutase to the Intracellular Survival of Clinical Isolates of Staphylococcus aureus in Murine Macrophages

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Abstract

The present study was performed in order to carefully investigate the interaction of Staphylococcus aureus with murine macrophages and the contribution of catalase and superoxide dismutase in intracellular persistence of Staphylococcus aureus within murine macrophages during in vitro infection. We have reported that Staphylococcus aureus internalized by murine macrophages did not appear to be rapidly killed. Data indicating the contribution of a single catalase and superoxide dismutase in intracellular survival of Staphylococcus aureus were provided using established biochemical assays. The results of the present experiment suggest that the survival of Staphylococcus aureus within phagocytic cells is facilitated by its ability to resist oxidative products. Organisms in the log phase of growth clearly demonstrate a resistance to oxidative products.

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Acknowledgments

This study was carried out with support of an unrestricted research grant from Indian Council of Medical Research (ICMR), New Delhi, Government of India, provided to Dr. Biswadev Bishayi, Senior Lecturer, Department of physiology, University of Calcutta, Kolkata. We are deeply indebted to Prof. (Mrs.) Manjusree Bal, Department of Physiology, University of Calcutta, for supplying us with S. aureus (CMC-524, ICH-629 and ICH-757). We remain thankful to Prof. Sanghamitra Raha, Saha Institute of Nuclear Physics, Kolkata, India, for their kind help and guidance in the in situ staining techniques.

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  1. Department of Physiology, Immunology Laboratory, University of Calcutta, 92, APC Road, Kolkata, 700009, West Bengal, India

    Debaditya Das & Biswadev Bishayi

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  1. Debaditya Das
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  2. Biswadev Bishayi
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Correspondence to Biswadev Bishayi.

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Das, D., Bishayi, B. Contribution of Catalase and Superoxide Dismutase to the Intracellular Survival of Clinical Isolates of Staphylococcus aureus in Murine Macrophages. Indian J Microbiol 50, 375–384 (2010). https://doi.org/10.1007/s12088-011-0063-z

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  • DOI: https://doi.org/10.1007/s12088-011-0063-z

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