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Establishment of lacZ marked strain of phosphate solubilizing bacterium in the rhizosphere and its effect on plant growth in mungbean

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Abstract

The establishment of lacZ marked strain of P-solubilizing bacterium Pseudomonas in the rhizosphere of mungbean (Vigna radiata) under pothouse conditions was studied. The lacZ marker was transferred to Pseudomonas P-36 on LB medium using donor strain of E. coli. The lacZ marked strain formed blue colonies on selective media and could be identified from soil on the basis of this character. The lacZ marked strain was able to survive in rhizosphere of mungbean under pothouse conditions and maintained a population of about 104 g−1 of rhizosphere soils up to 60 days study period. Positive effect of inoculation with P-solubilizing bacterium on dry matter yield, P and N-uptake was observed using rock phosphate and single super phosphate as P sources with and without farmyard amendment.

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Authors and Affiliations

  1. Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125 004, India

    S. Sunita, K. K. Kapoor, S. Goyal & P. K. Sharma

Authors
  1. S. Sunita
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  2. K. K. Kapoor
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  3. S. Goyal
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  4. P. K. Sharma
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Correspondence to K. K. Kapoor.

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Sunita, S., Kapoor, K.K., Goyal, S. et al. Establishment of lacZ marked strain of phosphate solubilizing bacterium in the rhizosphere and its effect on plant growth in mungbean. Indian J Microbiol 50 (Suppl 1), 117–121 (2010). https://doi.org/10.1007/s12088-010-0068-z

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  • DOI: https://doi.org/10.1007/s12088-010-0068-z

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