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Hypochlorite digestion method for efficient recovery of PHB from Alcaligenes faecalis

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Abstract

We reported the optimum amount of PHB accumulated by Alcaligenes faecalis during its 24 h growth under nitrogen deficient conditions. After 24 h incubation decrease in the amount of PHB was recorded. Hypochlorite digestion of biomass of organism followed by extraction with a solvent system consisting of 1:1 mixture of ethanol and acetone resulted in efficient recovery of PHB vis-à-vis earlier methods. This solvent system gave a high recovery yield, i.e. 5.6 gL−1 vis-à-vis earlier reported yield, 1.34 gL−1 (by same method), 0.63 gL−1 (by chloroform extraction method) and 1.1 gL−1 (by dispersion method).

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Authors and Affiliations

  1. Department of Microbiology, PSGVP Mandal’s ASC College Shahada, Maharashtra, 425 409, India

    R. Z. Sayyed & N. S. Gangurde

  2. Department of Microbiology, School of Life Sciences, North Maharashtra University, Jalgaon, 425 001, India

    S. B. Chincholkar

Authors
  1. R. Z. Sayyed
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  2. N. S. Gangurde
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  3. S. B. Chincholkar
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Correspondence to R. Z. Sayyed.

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Sayyed, R.Z., Gangurde, N.S. & Chincholkar, S.B. Hypochlorite digestion method for efficient recovery of PHB from Alcaligenes faecalis . Indian J Microbiol 49, 230–232 (2009). https://doi.org/10.1007/s12088-009-0036-7

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  • DOI: https://doi.org/10.1007/s12088-009-0036-7

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