Abstract
The present study was undertaken to study the immune response in calves vaccinated with Brucella abortus strain 19, infectious bovine rhinotracheitis (IBR) vaccines in monovalent form and combined vaccine containing both antigen. The seroconversion of monovalent and combined vaccines was tested in seronegative cattle calves. IBR vaccine alone and combination with live Brucella abortus S19 vaccine elicited an anamnestic response on day 60 post booster but started declining from day 90 onwards against IBR. B. abortus S19 alone and in combination with IBR vaccine gave more than 2 log protection in mice two weeks post challenge. Fluorescence polarization assay analysis with sera samples of calves vaccinated with B. abortus S19 monovalent vaccine alone and in combination with IBR vaccine revealed the presence of B. abortus antibodies. The components of the combined vaccine did not show any evidence of interference in the development of immunity. This combined vaccine may provide economical and affordable biological for the control of brucellosis and IBR.
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References
Moriyon I, Grillo MJ, Monreal D, Gonzalez D, Marin C, Lopez-goni I, Mainar-Jaime R, Moreno E and Blasco JM (2004) Rough vaccines in animal brucellosis: structural and genetic basis and present status. Vet Res 35:1–38
Nicoletti P (1990) Vaccination In: Nielsen, K, Duncan, JR (eds), Animal Brucellosis. CRC Press, Boca Raton, pp 284–299
Peters AR, Thevesagayam SJ, Wiseman A and Salt JS (2004) Duration of immunity of a quadrivalent vaccine against respiratory diseases caused by BHV-1, PI3V,BVDV and BRSV in experimentally infected calves. Prev Vet Med 66:63–77
Alton GG (1988) Techniques for the Brucellosis Laboratory, INRA, Paris, France
OIE Manual of Standards for Diagnostic Tests and Vaccines (2008) Bovine Brucellosis OIE, Paris, pp 624–659
Nielsen K and Gall D (2001) Fluorescence polarization assay for the diagnosis of brucellosis: a review. J Immunoassay 22:183–201
Karber G (1931) Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exptl Pathol Pharmakol 162:480–483
Schipper IA and Kelling CL (1975) Evaluation of inactivated infectious bovine rhinotracheitis vaccines. Can J Comp Med 32:412–415
Kilari S, Singh SN, Buche AV, Sawarkar S and Ghosh S (2000) Evaluation of immunogenicity of infectious bovine rhinotracheitis vaccine in Indian tropical climate. Indian Vet J 77:185–188
Stevens MG, Hennager SG, Olsen SC and Cheville NF (1994) Serologic responses in diagnostic tests for Brucellosis in cattle vaccinated with Brucella abortus 19 or RB51. J Clin Microbiol 32:1065–1066
Aguirre NP, Vanzini VR, Torioni de Echaide S, Valentini BS, De Lucca G, Aufranc C, Canal A, Vigliocco A and Nielsen K (2002) Antibody dynamics in Holstein Friesian heifers vaccinated with Babortus S19, using seven serological tests. J Immunoassay Immunochem 23(4):471–478
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Kamaraj, G., Chinchkar, S.R., Rajendra, L. et al. A combined vaccine against Brucella abortus and infectious bovine rhinotracheitis. Indian J Microbiol 49, 161–168 (2009). https://doi.org/10.1007/s12088-009-0028-7
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DOI: https://doi.org/10.1007/s12088-009-0028-7