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Biodegradation of γ-hexachlorocyclohexane (Lindane) by a non-white rot fungus conidiobolus 03-1-56 isolated from litter

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Abstract

Biodegradation of chlorinated pesticide γ-hexachlorocyclohexane (lindane) by a nonwhite rot fungus Conidiobolus 03-1-56 is reported for the first time. Conidiobolus 03-1-56, a phycomyceteous fungus isolated from litter, completely degraded lindane on the 5th day of incubation in the culture medium, and GC-ECD studies confirmed that lindane removal did not occur via adsorption on the fungal biomass. Degradation studies using different medium compositions showed that nitrogen/carbon limiting conditions (stress conditions) and presence of veratryl alcohol, induced the secretion of extracellular oxidative enzymes, which enhanced the rate of lindance biodegradation. Under optimum nutrient-limiting conditions, GC-ECD and GC-MS analysis showed complete absence of any degradation metabolite, indicating that lindane was completely mineralized. Assays for tannic acid utilization and lignin peroxidase showed similar enzymatic profiles between Conidiobolus 03-1-56 and standard white rot fungi Pleurotus ostreatus 1200 and Trametes versicolor 1086. Although Conidiobolus 03-1-56 showed a reduced enzyme activity compared to white rot fungi, preliminary evidence indicates that enzymes responsible for lignin degradation by white rots play a key role in lindane degradation by Conidiobolus 03-1-56.

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Authors and Affiliations

  1. Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India

    Varima Nagpal, M. C. Srinivasan & K. M. Paknikar

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  1. Varima Nagpal
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  2. M. C. Srinivasan
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  3. K. M. Paknikar
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Correspondence to K. M. Paknikar.

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Nagpal, V., Srinivasan, M.C. & Paknikar, K.M. Biodegradation of γ-hexachlorocyclohexane (Lindane) by a non-white rot fungus conidiobolus 03-1-56 isolated from litter. Indian J Microbiol 48, 134–141 (2008). https://doi.org/10.1007/s12088-008-0013-6

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  • DOI: https://doi.org/10.1007/s12088-008-0013-6

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