Skip to main content

Paddy straw as substrate for ethanol production

Abstract

Pretreatment of paddy straw with 2% sodium hydroxide at 15 psi for 1 h resulted in 83% delignification. The hydrolysis of alkali treated paddy straw with a commercial preparation of cellulase for 2 h at 50°C resulted in release of 65% total reducing sugars. Maximum sugars were released at enzyme loading of 1.5% (v/v). The fermentation of hydrolysate supplemented with nutrients by S. cerevisiae resulted in the production of 20–30 g L−1 ethanol after 48 h incubation which was further improved with addition of yeast nitrogen base and inoculated with 1% (w/v) yeast cells.

This is a preview of subscription content, access via your institution.

References

  1. Wheals AE, Barso LC, Alves DMG & Amorim HV (1999) Fuel ethanol after 29 years. Trends Biotechnol 17: 482–487.

    PubMed  Article  CAS  Google Scholar 

  2. Ward OP (2002) Bioethanol technology development and Perspective. Adv Appl Microbiol 51:53–80.

    PubMed  CAS  Article  Google Scholar 

  3. Ballerini D, Desmarquest JP & Pourquic J (1994) Ethanol production from lignocellulosics: large scale experimentation and economics. Bioresource Technol 50:14–23.

    Article  Google Scholar 

  4. Lee J (1997) Biological conversion of lignocellulosic biomass to ethanol. J Biotechnol 56:1–24.

    PubMed  Article  CAS  Google Scholar 

  5. Annonymous (1970) Official Methods of Analysis. Assoc. Official Agri Chemists 11th edn Washington, DC.

  6. Ghosh, TK (1987) Measurement of cellulase activities. Pure Appl chem 59:257–268.

    Google Scholar 

  7. Miller GL (1959) Use of di nitro salicylic acid reagent for estimation of reducing sugars. Anal Chem 31:426–428.

    Article  CAS  Google Scholar 

  8. Caputi P, Vede JM & Brown T (1968) Spectrophotometric determination of chromic complex formed during oxidation of ethanol. Am J Enol Vitic 19:1601–1665.

    Google Scholar 

  9. Schell JS, Riley CJ, Dowe N, Farmer J, Ibsen KN, Ruth MI, Toon ST & Lumpkin RI (2004) A bioethanol process development unit: initial operating experiences and results with a corn fiber feedstock. Bioresource Technol 91:179–188.

    Article  CAS  Google Scholar 

  10. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M & Ladish M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technol 9:637–686.

    Google Scholar 

  11. Sharma SK, Kalra KL & Kocher GS (2004) Fermentation of enzymatic hydrolysate of Sunflower hulls for ethanol production and its scale up. Biomass Bioenergy 27:399–402.

    Article  CAS  Google Scholar 

  12. Peiris PS & Silva I (1987) Hydrolysis of rice straw to fermentable sugar by Trichoderma enzymes. MIRCEN J 3:57–65.

    Article  CAS  Google Scholar 

  13. Vlasenko E Yu, Ding H, Labavitch JM & Shoemaker SP (1997) Enzymatic hydrolysis of pretreated rice straw. Bioresource Technol 59: 109–119.

    Article  CAS  Google Scholar 

  14. Ahring BK, Licht D, Schmidt AS & Sommer P (1999) Production of ethanol from wet oxidized wheat straw by Thermoanaerobacter mathranii. Bioresource Technol 68: 3–9.

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to L. Wati.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wati, L., Kumari, S. & Kundu, B.S. Paddy straw as substrate for ethanol production. Indian J Microbiol 47, 26–29 (2007). https://doi.org/10.1007/s12088-007-0005-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-007-0005-y

Key words

  • Cellulose
  • ethanol
  • fermentation
  • paddy straw
  • S. cerevisiae