Bone destruction in patients with chronic suppurative otitis media (CSOM) and cholesteatoma is considered to be quite high. Bone destruction is caused by various inflammatory cytokines and osteoclasts including IL-1α and TNF-α. The imbalance between the resorption process by osteoclasts and the process of bone formation is also a causative factor for bone destruction. On top of that, the large number of patients is not supported by an equal amount of medical facilities and personnel to conduct operative procedures. To analyze the associated of IL-1α level and TNF-α expression on the severity of bone destruction in CSOM and cholesteatoma patients. The total number of the subjects was 46 patients which group I (TNF-α) consisted of 26 individuals and group II (IL-1α) contained 26 individuals as well. The analysis was conducted in 2 different places (Solo, Indonesia and Surabaya, Indonesia). IL-1α expression was assessed by using ELISA kit at the absorbance rate of 450 nm whereas the rabbit anti-TNF-α polyclonal antibody was applied to examine TNF-α. The assessment of bone destruction was carried out during the operative procedure in Dr. Soetomo General Hospital Surabaya, Indonesia. Group I assessment resulted in severe bone destruction of 65.39% whilst group II showed severe bone destruction of 65.00%. This study revealed that TNF-α was categorized as strong positive (34.62%), moderate positive (42.30%), weak positive (19.23%), and negative (3.85%) with the value of r = 0.775; p ≤ 0.001. On the other hand, the rate of IL-1α was attained as follows: 14.93 ± 4.36 pg/ml, 22.75 ± 12.18 pg/ml, and 31.98 ± 14.16 pg/ml with the value of r = 0.625; p = 0.003. There is a significant association between expression of TNF-α and IL-1α level on the severity of bone destruction in CSOM and cholesteatoma patients. Hence, it has been proven that it is necessary to develop an additional therapeutic interventions to reduce TNF-α and IL-1α in CSOM and cholesteatoma patients.
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Datta G, Baisakhiya N, Mendiratta V (2014) Unsafe CSOM still a challenge in rural areas. Otolaryngol Online J 4(2):1–16
Xie S, Wang X, Ren J, Liu W (2017) The role of bone resorption in the etiopathogenesis of acquired middle ear cholesteatoma. Eur Arch Oto-Rhino-Laryngol 274(5):2071–2078
Yao Z, Xing L, Qin C, Schwarz EM, Boyce BF (2008) Osteoclast precursor interaction with bone matrix induces osteoclast formation directly by an interleukin-1-mediated autocrine mechanism. J Biol Chem 283(15):9917–9924
Kim JH, Jin HM, Kim K, Song I, Youn BU, Matsuo K et al (2009) The mechanism of osteoclast differentiation induced by IL-1. J Immunol 183(3):1862–1870
Kuczkowski J, Sakowicz-Burkiewicz M, Iżycka-Świeszewska E, Mikaszewski B, Pawełczyk T (2011) Expression of tumor necrosis factor-α, interleukin-1α, interleukin-6 and interleukin-10 in chronic otitis media with bone osteolysis. ORL 73(2):93–99
Lee Y-M, Fujikado N, Manaka H, Yasuda H, Iwakura Y (2010) IL-1 plays an important role in the bone metabolism under physiological conditions. Int Immunol 22(10):805–816
Kuczkowski J, Sakowicz-Burkiewicz M, Iżycka-Świeszewska E (2010) Expression of the receptor activator for nuclear factor-κB ligand and osteoprotegerin in chronic otitis media. Am J Otolaryngol 31(6):404–409
Jung JY, Chole RA (2002) Bone resorption in chronic otitis media: the role of the osteoclast. ORL 64(2):95–107
Anggraeni R, Hartanto WW, Djelantik B, Ghanie A, Utama DS, Setiawan EP et al (2014) Otitis media in Indonesian urban and rural school children. Pediatr Infect Dis J 33(10):1010–1015
Park M, Lee JS, Lee JH, Oh SH, Park MK (2015) Prevalence and risk factors of chronic otitis media: the Korean National Health and Nutrition Examination Survey 2010–2012. PloS One 10(5):e0125905-e
Artono A, Purnami N, Rahmawati R (2016) Biofilm bacteria plays a role in csom pathogenesis and has significant correlation with unsafe type CSOM. Folia Med Indones 51(4):208–213
Zhao X, Fan W, Xu Z, Chen H, He Y, Yang G et al (2016) Inhibiting tumor necrosis factor-alpha diminishes desmoplasia and inflammation to overcome chemoresistance in pancreatic ductal adenocarcinoma. Oncotarget 7(49):81110–81122
Aruan SY, Aboet A, Zahara D, Aliandri A, Saragih AR (2015) Hubungan ekspresi tumor necrosis factor alpha (TNF-α) dengan destruksi tulang akibat kolesteatoma. Oto Rhino Laryngol Indones 45(1):36–42
Dretzke J, Edlin R, Round J, Connock M, Hulme C, Czeczot J et al (2011) A systematic review and economic evaluation of the use of tumour necrosis factor-alpha (TNF-α) inhibitors, adalimumab and infliximab, for Crohn's disease. Health Technol Assess 15(6):1
Coates LC, Marzo-Ortega H, Bennett AN, Emery P (2010) Anti-TNF therapy in ankylosing spondylitis: insights for the clinician. Ther Adv Musculoskelet Dis 2(1):37–43
Akimoto R, Pawankar R, Yagi T, Baba S (2000) Acquired and congenital cholesteatoma: determination of tumor necrosis factor-alpha, intercellular adhesion molecule-1, interleukin-1-alpha and lymphocyte functional antigen-1 in the inflammatory process. ORL 62(5):257–265
Moltó A, Olivé A (2010) Anti-IL-1 molecules: new comers and new indications. Joint Bone Spine 77(2):102–107
Dinarello CA, Simon A, van der Meer JWM (2012) Treating inflammation by blocking interleukin-1 in a broad spectrum of diseases. Nat Rev Drug Discov 11(8):633–652
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Artono, Surarto, B., Purnami, N. et al. The Association of IL-1 Alpha Level and TNF Alpha Expressions on Bone Destruction in Chronic Suppurative Otitis Media and Cholesteatoma. Indian J Otolaryngol Head Neck Surg 72, 1–7 (2020). https://doi.org/10.1007/s12070-019-01704-z
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