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Matrix Metalloproteinase-9 Expression and Status of Cervical Lymph Nodes in Patients with Nasopharyngeal Carcinoma

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Abstract

Tumor growth and metastasis in nasopharyngeal carcinoma (NPC) patients was suspected as a role of several molecular biomarkers that have been identified in tumor specimens of patients with NPC. Invasion and metastasis process was a complex mechanism which involved some proteolytic enzymes, such as matrix metalloproteinase-9 (MMP-9). To analyze the association of MMP-9 expression of NPC patients with cervical lymph node metastasis. The study was conducted in oncology unit of ORL-HNS at Dr. Soetomo General Hospital Surabaya from May to July 2015. Formalin-fixed paraffin-embedded biopsy specimens from NPC patients with WHO type II and III of histopathology and clinically were divided into four state of cervical enlargement (N0. N1. N2 and N3). The expression of MMP-9 was obtained with immunohistochemistry using rabbit polyclonal antibody Anti-MMP9 ab7299 from abcam®. Cambridge-UK. Thirty-two NPC patients were enrolled in this study. The study found a negative expression of MMP-9 in 3.12% of samples. Spearman rho test result was p = 0.001 with correlation coefficient of 0.754. Spearman test resulted p value of 0.001 with a correlation coefficient of 0.754. Correlation of matrix metalloproteinase-9 expression with cervical lymph node metastasis (N0, N1, N2, and N3) in patients with NPC showed a significant result (p < 0.05). There was a strong positive correlation between MMP-9 expressions with cervical lymph node status in NPC patients.

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References

  1. Kentjono WA (2010) Karsinoma nasofaring: etiologi, gejala, diagnosis, deteksi dini, terapi dan pencegahan. In: Kentjono WA (ed) Pelatihan deteksi dini kanker nasofaring untuk dokter umum di puskesmas. Dept/SMF Ilmu Kesehatan THT-KL FK Unair/RSUD Dr. Soetomo, Surabaya, pp 13–41

    Google Scholar 

  2. Horikawa T Yoshizaki T, Sheen TS, Lee SY, Furukawa M, et al (2000) Association of LMP 1 and MMP 9 with metastasis in NPC. In: Japan-Taiwan conference. Japan: American Cancer Society. vol 89, pp 715–23

  3. Wakisaka N, Wen Q, Yoshizaki T, Furukawa M, Kawahara M, Nakanishi I et al (1999) Association of vascular endhothelial growth factor expression with angiogenesis and lymph node metastasis in nasopharyngeal carcinoma. The Laryngoscope 109:810–814

    Article  CAS  Google Scholar 

  4. Liu ZLL, Yang Z, Luo W, Li X, Yang H et al (2010) Increased expression of MMP 9 is correlated with poor prognosis of nasopharyngeal carcinoma. BMC Cancer J 10:1–7

    Article  CAS  Google Scholar 

  5. Astuti AY (2014) Ekspresi E-Cadherin dan MMP-9 pada karsinoma nasofaring tanpa metastasis, dengan metastasis awal dan metastasis lanjut kelenjar getah bening. Dalam Karya Akhir untuk memperoleh ijazah keahlian Ilmu Patologi Anatomi. Departemen/SMF Patologi Anatomi FKUA/RSUD Dr. Soetomo Surabaya

  6. He JR, Qin H, Ren ZF et al (2011) MMP-9 expression in peripheral blood mononuclear cells and the association with clinicopathological features and prognosis of nasopharyngeal carcinoma. Clin Chem Lab Med 49:705–710

    CAS  PubMed  Google Scholar 

  7. Vilen ST, Salo T, Sorsa T, Nyberg P (2013) Fluctuating roles of matrix metalloproteinase-9 in oral squamous cell carcinoma. Sci World J. https://doi.org/10.1155/2013/920595

    Article  Google Scholar 

  8. Harahap MPH (2009) Ekspresi vascular endothelial growth factor pada karsinoma nasofaring. Universitas Sumatra Utara Repository, Medan

    Google Scholar 

  9. Mulyarjo (2002) Diagnosis dan penatalaksanaan karsinoma nasofaring. In: Mulyarjo D, Soedjak S, Wisnubroto W, Harmadji S, Hasanusi S, Artono R (eds) Naskah Lengkap Pendidikan Kedokteran Berkelanjutan III Ilmu Kesehatan THT-KL. Laboratorium/SMF Ilmu Penyakit THT-KL FK Unair/RSUD Dr. Soetomo, Surabaya, pp 38–48

  10. Sukardja IDG (2000) Onkologi klinik. 2nd edn. Airlangga University Press, Surabaya, pp 239–256

  11. Zeng MS (2010) Pathogenesis and etiology of nasopharyngeal carcinoma. In: Cooper JS, Lu JJ, Lee AWM (eds) Nasopharyngeal cancer multidisciplinary management. Springer, Berlin, pp 12–20

    Google Scholar 

  12. Chang ET, Adami HO (2006) The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomark Prev Publ Am Assoc Cancer Res Cosponsored Am Soc Prev Oncol 15:1765–1777

    Article  CAS  Google Scholar 

  13. Ma JCS (2010) The epidemidemiology of nasopharyngeal cancer. In: Cooper JS, Lu JJ, Lee AWM (eds) Nasopharyngeal cancer multidisciplinary management. Springer, Berlin, pp 2–6

    Google Scholar 

  14. Li WRR, Gao DL, Fitzgibbons ED, Seixos NS, Camp JE, Wernli KJ, Astrakianakis G, Feng Z, Thomas DB, Checkoway H (2005) Occupational risk factors for nasopharyngeal cancer among female textile workers in Shanghai China. Occup Environ Med 63:39–44

    Article  Google Scholar 

  15. Soetjipto D (1989) Karsinoma nasofaring. Balai Penerbit Fakultas Kedokteran Universitas Indonesia, Jakarta, pp 71–84

    Google Scholar 

  16. Petruzelli GJ (2000) The biology of tumor invasion, angiogenesis and lymph node metastasis. J Otorhinolaryngol Relat Spec 64:178–185

    Article  Google Scholar 

  17. Yoon SOPS, Yun CH, Chung AS et al (2003) Roles of matrix metalloproteinase in tumor metastasis and angiogenesis. J Biochem Mol Biol 36:128–137

    CAS  PubMed  Google Scholar 

  18. Rundhaug JE (2003) Matrix metalloproteinases, angiogenesis, and cancer: commentary re: A. C. Lockhart, Reduction of wound angiogenesis in patients treated with BMS-275291, a broad spectrum matrix metalloproteinase inhibitor. Clin. Cancer Res., 9: 00–00. Clin Cancer Res Off J Am Assoc Cancer Res 2003(9):551–554

    Google Scholar 

  19. Murawski M, Wozniak M, Dus-Szachniewicz K, Kolodziej P, Rzeszutko M, Ziolkowski P (2016) Significance of matrix metalloproteinase 9 expression as supporting marker to cytokeratin 19 mRNA in sentinel lymph nodes in breast cancer patients. Int J Mol Sci 17:1–13

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga - Dr, Soetomo General Hospital, Jalan Mayjen Prof. Dr. Moestopo No. 47, Surabaya, 60131, Indonesia

    Muhtarum Yusuf, Yuan Ariawan Kusuma & Dwi Reno Pawarti

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  1. Muhtarum Yusuf
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  2. Yuan Ariawan Kusuma
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Correspondence to Muhtarum Yusuf.

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Yusuf, M., Kusuma, Y.A. & Pawarti, D.R. Matrix Metalloproteinase-9 Expression and Status of Cervical Lymph Nodes in Patients with Nasopharyngeal Carcinoma. Indian J Otolaryngol Head Neck Surg 71 (Suppl 1), 637–642 (2019). https://doi.org/10.1007/s12070-018-1451-z

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  • DOI: https://doi.org/10.1007/s12070-018-1451-z

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