Skip to main content

Advertisement

SpringerLink
Log in

Efficiency of Cryotherapy in Avoiding Myringotomy Closure in Rats

  • Original Article
  • Published:
Indian Journal of Otolaryngology and Head & Neck Surgery Aims and scope Submit manuscript

Abstract

Myringotomy and ventilation tube placement is an office-based procedure and represents the treatment of choice for middle ear effusions and for transtympanic drug administration. The aim of our study was to study the efficiency of low-pressure spray cryotherapy on protraction of patency time of myringotomies in rats. Bilateral myringotomies were performed with cold instruments. Afterward, one ear in each rat was randomly assigned for liquid nitrogen treatment of the perforated eardrum. Myringotomy patency was recorded daily and histological analysis of the eardrums was performed. In the myringotomy group patency time was 4.75 ± 2.93 days, while in the cryotherapy group was 11.01 ± 4.87 days. The difference is statistically significant: Student t test, p < 0.00001, 95 % CI (−8.82; −3.67). Moreover, according to Kaplan–Meier method, myringotomy survival is significantly longer in the cryotherapy group. The healing processes of the cryotreated eardrums last longer and the patency of myringotomy was longer as compared to untreated myringotomies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Bluestone CD, Klein JO (1996) Otitis media, atelectasis, and eustachian tube dysfunction. In: Bluestone CD, Stool S (eds) Pediatric otolaryngology, 3rd edn. WB Saunders Co, Philadelphia, pp 540–548

    Google Scholar 

  2. Ah-Tye C, Paradise JL, Colborn DK (2001) Otorrhea in young children after tympanostomy-tube placement for persistent middle-ear effusion: prevalence, incidence, and duration. Pediatrics 107(6):1251–1258

    Article  CAS  PubMed  Google Scholar 

  3. Jassir D, Buchman CA, Gomez-Marin O (2001) Safety and efficacy of topical mitomycin C in myringotomy patency. Otolaryngol Head Neck Surg 124:368–373

    Article  CAS  PubMed  Google Scholar 

  4. Gocea A, Taulescu M, Trombitas V, Albu S (2013) Effects of cryotherapy on the maxillary antrostomy patency in a rabbit model of chronic rhinosinusitis. Biomed Res Int 2013:101534. doi:10.1155/2013/101534

    Article  PubMed Central  PubMed  Google Scholar 

  5. Somers T, Goovaerts G, Schelfhout L, Peeters S, Govaerts PJ, Offeciers E (1998) Growth factors in tympanic membrane perforations. Otol Neurotol 19(4):428–434

    CAS  Google Scholar 

  6. Boedts D, Ars B (1977) Histopathological research on eardrum perforations. Arch otorhinolaryngol 215(1):55–59

    Article  CAS  PubMed  Google Scholar 

  7. Makino K, Amatsu M, Kinishi M, Mohri M (1990) Epithelial migration in the healing process of tympanic membrane perforations. Eur Arch Otorhinolaryngol 247(6):352–355

    Article  CAS  PubMed  Google Scholar 

  8. Ma Y, Zhao H, Zhou X (2002) Topical treatment with growth factors for tympanic membrane perforations: progress towards clinical application. Acta Otolaryngol 122(6):586–599

    Article  CAS  PubMed  Google Scholar 

  9. Larson TR, Rrobertson DW, Corica A, Bostwick DG (2000) In vivo interstitial temperature mapping of the human prostate during cryosurgery with correlation to histopathologic outcomes. Urology 55(4):547–552

    Article  CAS  PubMed  Google Scholar 

  10. Gage AA, Baust J (1998) Mechanisms of tissue injury in cryosurgery. Cryobiology 37(3):171–186

    Article  CAS  PubMed  Google Scholar 

  11. Goertz O, Baerreiter S, Ring A, Jettkant B, Hirsch T, Daigeler A, Langer S (2011) Determination of microcirculatory changes and angiogenesis in a model of frostbite injury in vivo. J Surg Res 168(1):155–161

    Article  PubMed  Google Scholar 

  12. Pimente CB, Moraes AMD, Cintra ML (2014) Angiogenic effects of cryosurgery with liquid nitrogen on the normal skin of rats, through morphometric study. Anais brasileiros de dermatologia 89(3):410–413

    Article  Google Scholar 

  13. Kardos TB, Ferguson MM (1991) Comparison of cryosurgery and the carbon dioxide laser in mucosal healing. Int J Oral Maxillofac Surg 20(2):108–111

    Article  CAS  PubMed  Google Scholar 

  14. Carmeliet P (2001) Clotting factors build blood vessels. Science 293(5535):1602–1604

    Article  CAS  PubMed  Google Scholar 

  15. Carmeliet P (2003) Angiogenesis in health and disease. Nat Med 9(6):653–660

    Article  CAS  PubMed  Google Scholar 

  16. Estrem SA, Batra PS (1999) Preventing myringotomy closure with topical mitomycin C in rats. Otolaryngol Head Neck Surg 120(6):794–798

    Article  CAS  PubMed  Google Scholar 

Download references

Conflict of Interest

The authors declare that they have no conflict of interest. No funding was received for this study.

Author information

Authors and Affiliations

  1. ENT Department, Military Hospital, Cluj-Napoca, Romania

    Felician Chirteş

  2. Otolaryngology Head and Neck Surgery Department, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Veronica Trombitas, Diana Vlad & Silviu Albu

  3. Pathology Department, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

    Flaviu Tabaran

  4. Department of Experimental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Cristian Berce

  5. II-nd Department of Otolaryngology, University of Medicine and Pharmacy Cluj-Napoca, Str. Republicii nr. 18, 400015, Cluj-Napoca, Romania

    Silviu Albu

Authors
  1. Felician Chirteş
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Veronica Trombitas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Diana Vlad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Flaviu Tabaran
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cristian Berce
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Silviu Albu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Silviu Albu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chirteş, F., Trombitas, V., Vlad, D. et al. Efficiency of Cryotherapy in Avoiding Myringotomy Closure in Rats. Indian J Otolaryngol Head Neck Surg 67, 68–71 (2015). https://doi.org/10.1007/s12070-014-0782-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12070-014-0782-7

Keywords

Search

Navigation