Abstract
Background
Decellularization methods using anionic detergents have been applied to engineered tissues for eliminating tissue immunogenicity. Sodium deoxycholate is a water soluble ionic detergent commonly used in applications ranging from decellularisation, liposome preparation and isolation of membrane proteins and lipids. Sodium deoxycholate has been reported to form many reactive intermediates which bind to tissue and exert toxic effects.
Methods
This work reports on methods to detect and estimate residual sodium deoxycholate in decellularized saphenous vein. The method reported is based on the extraction of anionic detergent (sodium deoxycholate)-Methylene blue complex in chloroform which can be subsequently quantified by measuring the optical density at 653 nm.
Results and conclusion
This assay may be used for detecting sodium deoxycholate levels in decellularized tissue before implantation.
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Acknowledgements
The authors wish to thank R. Balasundari and Sheerin Begam Nasser for help with decellularisation experiments, Dillip Kumar Bishi, Suneel Ralapalli, Kavitha Sankaranarayanan for scientific input and Pandian A. for technical assistance.
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Mathapati, S., Galla, S., Sankaranarayanan, K. et al. Qualitative and quantitative detection of sodium deoxycholic acid in decellularized tissue. Indian J Thorac Cardiovasc Surg 26, 129–131 (2010). https://doi.org/10.1007/s12055-010-0016-x
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DOI: https://doi.org/10.1007/s12055-010-0016-x