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Synchrotron-based X-ray fluorescence, imaging and elemental mapping from biological samples

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Abstract.

The present study utilized the new hard X-ray microspectroscopy beamline facility, X27A, available at NSLS, BNL, USA, for elemental mapping. This facility provided the primary beam in a small spot of the order of ∼10 μm, for focussing. With this spatial resolution and high flux throughput, the synchrotron-based X-ray fluorescent intensities for Mn, Fe, Zn, Cr, Ti and Cu were measured using a liquid-nitrogen-cooled 13-element energy-dispersive high-purity germanium detector. The sample is scanned in a ‘step-and-repeat’ mode for fast elemental mapping measurements and generated elemental maps at 8, 10 and 12 keV, from a small animal shell (snail). The accumulated trace elements, from these biological samples, in small areas have been identified. Analysis of the small areas will be better suited to establish the physiology of metals in specific structures like small animal shell and the distribution of other elements.

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Authors and Affiliations

  1. Department of Physics, Sir C R Reddy Autonomous College, Eluru, 534 007, India

    D V RAO

  2. Department of Physics, Sri Durga Malleswari Siddhartha Mahila Kalasala, Bunder Road, Vijayawada, 520 010, India

    M SWAPNA

  3. Istituto di Matematica e Fisica, Università degli Studi di Sassari, Via Vienna 2, 07100, Sassari, Italy

    R CESAREO & A BRUNETTI

  4. Department of Bio-System Engineering, Faculty of Engineering, Yamagata University, Yonezawa, Japan

    T AKATSUKA & T YUASA

  5. Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan

    T TAKEDA

  6. Dipartimento di Fisica, Universita di Roma, “La Sapienza”, 00185, Roma, Italy

    G E GIGANTE

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  1. D V RAO
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  2. M SWAPNA
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  3. R CESAREO
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  4. A BRUNETTI
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  5. T AKATSUKA
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  6. T YUASA
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  7. T TAKEDA
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  8. G E GIGANTE
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Correspondence to D V RAO.

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RAO, D.V., SWAPNA, M., CESAREO, R. et al. Synchrotron-based X-ray fluorescence, imaging and elemental mapping from biological samples. Pramana - J Phys 76, 261–269 (2011). https://doi.org/10.1007/s12043-011-0031-5

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  • DOI: https://doi.org/10.1007/s12043-011-0031-5

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