Seed Coating and Rice Grain Stickiness

Abstract

The quality concept for cooked rice (Oryza sativa L.) varies according to customers culture and habits, varying from stickier, in Asia, to looser grains, more appreciated in South America. This parameter, no matter the taste, is an important factor in the evaluation of what constitutes a good rice grain. Higher grain stickiness is associated not only with lower amylose content, but also with amylopectin chain length and the net of proteins reinforced by intra and intermolecular disulfide bonds around the rice starch granule. Therefore, the present study compared four rice genotypes and the effectiveness of seed zinc coating treatments: T0 (without zinc sulfate) and TZnSO4 (with 8.81 g of ZnSO4.7H2O. kg−1 of seeds). Here we show that both treatments affect transcript accumulation of important sulfur-metabolism-enzymes. However, the higher levels of transcripts from these genes did not result in higher cysteine or serine levels. The adhesiveness of cooked mature rice grains is greatly impacted by genotype, but no evidence that transcript up-regulation could be associated to stickiness.

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Abbreviations

AACC:

American Association for Clinical Chemistry

APR:

APS REDUCTASE

APSK:

ATP GENERATING ADENOSINE 5C-PHOSPHOSULFATE KINASE

ATPS:

ATP SULFURYLASE

Capes:

Coordination for the Improvement of Higher Education Personnel

cDNA:

Complementary DNA

CNPq:

National Counsel of Technological and Scientific Development

DAA:

days after anthesis

HPLC:

High Performance Liquid Chromatography

OAS-TL:

O-ACETYLSERINE THIOL LYASE

RT qPCR:

Real-Time quantitative PCR

SAT:

SERINE ACETYLTRANSFERASE

SiR:

FERREDOXIN DEPENDENT SULFITE REDUCTASE

SOSBAI:

Sociedade Sul-Brasileira de Arroz Irrigado

UBQ5 :

Ubiquitin 5

ZnSO4 :

Zinc sulfate

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Acknowledgement

This study was supported by RiceTec Sementes Ltda, grant CGFTR02/2012. Scholarships were provided by Coordination for the Improvement of Higher Education Personnel (CAPES), Research Support Foundation of Rio Grande do Sul (FAPERGS) and The Brazilian National Council for Scientific, Technological Development (CNPq).

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da Costa, C.M., dos Santos, R.S., Marini, N. et al. Seed Coating and Rice Grain Stickiness. Tropical Plant Biol. 13, 225–235 (2020). https://doi.org/10.1007/s12042-019-09252-0

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Keywords

  • Cooking quality
  • Oryza sativa
  • RT-qPCR
  • Sulfate assimilation