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Identification, Characterization and Expression Profiles of Dof Transcription Factors in Pineapple (Ananas comosus L)

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Abstract

DNA binding with one finger (Dof) transcription factors (TF) family is a class of plant specific transcription factors and has crucial roles in development of different tissues and response to stresses. Using GWAS we identified twenty six Dof genes located on 16 pineapple chromosomes. Dof family genes were distributed into five groups and contain highly conserved motif. High expression of AcoDof1, AcoDof12, AcoDof26 (root), AcoDof9, AcoDof22, AcoDof19 (fruit), AcoDof23, AcoDof25 (ovule), AcoDof11, AcoDof17 and AcoDof20 (petal) were found in RNA-Seq analysis. RNA-Seq data also suggest that Dof genes could have important role in female gametophyte development of pineapple. Expression analysis using qRT-PCR of pineapple Dof genes family under different abiotic stress (cold, heat, salt, drought) showed a dynamic response of Dof genes. AcoDof1 was up-regulated at 48 h treatment in all four abiotic stresses, while AcoDof20 was up-regulated at 24 h of treatment. Moreover, AcoDof8, AcoDof12, AcoDof2 were up-regulated by salinity stress. Dof genes expression during abiotic stress reveals their vital role for pineapple growth and development which could be utilized agronomically. Present study provides a framework for future studies related to the functions of Dof genes in pineapple.

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Abbreviations

TFs:

Transcription factors

RNA-Seq:

RNA sequencing

qRT-PCR:

Quantitative real-time PCR

NaCl:

Sodium chloride

ORF:

Open reading frame

IP:

Iso-electric point

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Acknowledgments

This work was supported by NSFC (U1605212; 31522009 to Y.Q.), Fujian Innovative Center for Germplasm Resources and Innovation Project of Characteristic Horticultural Crop Seed Industry (KLA15001D) and FAFU international collaboration project (KXb16006A).

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Contributions

S.M. Azam performed experiments, data analysis and manuscript writing, Y.L carried out bioinformatics assistance, Z.R and H.A worked on qRT-PCR, L.W, B.H, X.H and X.J.J helped and observed RNA-Seq analysis. We are thankful to M. Aslam for reviewing the manuscript.. YQ conceived the study.

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Correspondence to Yuan Qin.

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The authors declare that they have no conflict of interests.

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Communicated by: Yann-Rong Lin

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Azam, S.M., Liu, Y., Rahman, Z.U. et al. Identification, Characterization and Expression Profiles of Dof Transcription Factors in Pineapple (Ananas comosus L). Tropical Plant Biol. 11, 49–64 (2018). https://doi.org/10.1007/s12042-018-9200-8

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  • DOI: https://doi.org/10.1007/s12042-018-9200-8

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