Evaluation of QTLs for Shoot Fly (Atherigona soccata) Resistance Component Traits of Seedling Leaf Blade Glossiness and Trichome Density on Sorghum (Sorghum bicolor) Chromosome SBI-10L

Abstract

Shoot fly is a major insect pest of sorghum damaging early crop growth, establishment and productivity. Host plant resistance is an efficient approach to minimize yield losses due to shoot fly infestation. Seedling leaf blade glossiness and trichome density are morphological traits associated with shoot fly resistance. Our objective was to identify and evaluate QTLs for glossiness and trichome density using- i) 1894 F2s, ii) a sub-set of 369 F2-recombinants, and iii) their derived 369 F2:3 progenies, from a cross involving introgression lines RSG04008-6 (susceptible) × J2614-11 (resistant). The QTLs were mapped to a 37–72 centimorgan (cM) or 5–15 Mb interval on the long arm of sorghum chromosome 10 (SBI-10L) with flanking markers Xgap001 and Xtxp141. One QTL each for glossiness (QGls10) and trichome density (QTd10) were mapped in marker interval Xgap001-Xnhsbm1044 and Xisep0630-Xtxp141, confirming their loose linkage, for which phenotypic variation accounted for ranged from 2.29 to 11.37 % and LOD values ranged from 2.03 to 24.13, respectively. Average physical map positions for glossiness and trichome density QTLs on SBI-10 from earlier studies were 4 and 2 Mb, which in the present study were reduced to 2 Mb and 800 kb, respectively. Candidate genes Glossy15 (Sb10g025053) and ethylene zinc finger protein (Sb10g027550) falling in support intervals for glossiness and trichome density QTLs, respectively, are discussed. Also we identified a sub-set of recombinant population that will facilitate further fine mapping of the leaf blade glossiness and trichome density QTLs on SBI-10.

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Acknowledgments

This work is a part of the Ph.D. thesis of KNSUK. Authors are grateful to Mr. C. Muralidhar for his help and support for conducting this complete study. This work was supported by ICRISAT’s Research Program on Dryland Cereals. This work was undertaken as part of the CGIAR Research Program on Dryland Cereals.

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Correspondence to Santosh P. Deshpande.

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Communicated by: Ray Ming

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Kiranmayee, K.N.S.U., Kishor, P.B.K., Hash, C.T. et al. Evaluation of QTLs for Shoot Fly (Atherigona soccata) Resistance Component Traits of Seedling Leaf Blade Glossiness and Trichome Density on Sorghum (Sorghum bicolor) Chromosome SBI-10L. Tropical Plant Biol. 9, 12–28 (2016). https://doi.org/10.1007/s12042-015-9157-9

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Keywords

  • Shoot fly
  • F2
  • F2:3
  • Leaf blade glossiness
  • Trichome density
  • QTLs