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Extraction of genomic DNA for sequencing from snail Helix lucorum

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Abstract

Genomic studies make it possible to breakthrough in many fields such as biochemistry, physiology, phylogenetics, etc., though they are unworkable without sequences of genomic DNA of an organism. The terrestrial mollusks’ genomes would benefit gastropod biology investigations, that are unavailable so far due to problems in DNA integrity and quality after the isolation procedures. Here we describe a fast and handy protocol for genomic DNA extraction from the tissues of Helix lucorum, which allows to yield high-quality samples applicable for downstream analysis such as high-throughput DNA sequencing. Troubleshooting revealed the nuclease activity of snail tissue lysate, which may be avoided by heating the lysate and decreasing the incubation time.

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Acknowledgements

We sincerely thank Dr P. M. Balaban for providing animals and inspiring the work.

Author information

Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology of Russian Academy of Sciences, Moscow, 117485, Russia

    Dmitry Panteleev, Anastasia Sadova & Galina Pavlova

  2. Burdenko National Medical Research Center of Neurosurgery, Ministry of Health of the Russian Federation, Moscow, 125047, Russia

    Galina Pavlova

  3. Sechenov First Moscow State Medical University, Moscow, 119435, Russia

    Galina Pavlova

Authors
  1. Dmitry Panteleev
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  2. Anastasia Sadova
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  3. Galina Pavlova
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by DP, and AS. The first draft of the manuscript was written by GP and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Anastasia Sadova.

Additional information

Ad-hoc editor: Jahnavi Joshi

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Panteleev, D., Sadova, A. & Pavlova, G. Extraction of genomic DNA for sequencing from snail Helix lucorum. J Genet 103, 20 (2024). https://doi.org/10.1007/s12041-024-01472-y

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  • DOI: https://doi.org/10.1007/s12041-024-01472-y

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