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Novel and stable QTL regions conferring resistance to MYMV disease and its inheritance in blackgram (Vigna mungo (L.) Hepper)

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Abstract

Mungbean yellow mosaic virus (MYMV) disease is a significant constraint for blackgram production. The present study employed a mapping population derived from a cross between susceptible (MDU 1) and resistant (TU 68) genotypes to identify quantitative trait loci (QTL) associated with MYMV disease resistance in addition to bruchine resistance loci identified from the previous study. Phenotyping was carried out in F2 generation under the disease spreader row method at field condition. Disease score observations were carried out 60 days after sowing (DAS). The chi-square goodness of fit test revealed inhibitory gene action with two genes controlling the expression of resistance to MYMV disease. However, QTL analysis revealed one major QTL region, i.e. qMYMVD_60 at LG 10 responsible for MYMV disease score at 60 DAS, accounted for 21 per cent of variation. The identified QTL has the flanking markers as CEDG180 and CEDG116. Hence, the QTL, qMYMVD_60 may be utilized in the breeding of MYMV disease resistance. Further, the marker-assisted introgression of both the MYMV and bruchine resistance QTLs can be performed in the near future.

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Acknowledgements

Authors acknowledge the help rendered by Mr Arul Doss, Agricultural Supervisor, NPRC, Vamban in the trial.

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Authors and Affiliations

  1. National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, 622 303, India

    Ragul Subramaniyan

  2. Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Ragul Subramaniyan & Manivannan Narayana

  3. Department of Millets, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Iyanar Krishnamoorthy

  4. Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Ganapathy Natarajan

  5. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Karthikeyan Gandhi

Authors
  1. Ragul Subramaniyan
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  2. Manivannan Narayana
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  3. Iyanar Krishnamoorthy
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  4. Ganapathy Natarajan
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  5. Karthikeyan Gandhi
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Contributions

SR performed the field experiments, measurements, data analysis and drafted the manuscript. NM supervised the work, worked on the manuscript and aided in interpreting the results. NM, KI, NG and GK were involved in planning. All authors provided critical feedback on research, analysis and manuscript.

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Correspondence to Manivannan Narayana.

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Corresponding editor: Qingpo Liu

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Subramaniyan, R., Narayana, M., Krishnamoorthy, I. et al. Novel and stable QTL regions conferring resistance to MYMV disease and its inheritance in blackgram (Vigna mungo (L.) Hepper). J Genet 101, 18 (2022). https://doi.org/10.1007/s12041-022-01359-w

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  • DOI: https://doi.org/10.1007/s12041-022-01359-w

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