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Identification of broad-spectrum resistance QTLs against rice blast fungus and their application in different rice genetic backgrounds

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Abstract

Rice blast disease is found worldwide leading to economic losses. Use of resistance gene is effective to improve rice resistance variety. Therefore, to deploy genomic regions harbouring resistance genes, a population of 587 F2:6 recombinant inbred lines (RILs) was developed from a cross between Jao Hom Nin, a Thai black rice variety with broad-spectrum resistance to blast disease, and Kao Dawk Mali 105, a susceptible Thai jasmine variety. The RILs were challenged with 17 blast isolates collected from Thailand and Laos PDR. Quantitative trait locus analysis identified genomic regions associated with broad-spectrum quantitative resistance (qBSRLs) and race-specific quantitative resistance (qRSRLs). Two qBSRLs were detected on chromosomes 1 and 11, and two qRSRLs were detected on chromosomes 8 and 12. The two qBSRLs were introgressed into two new genetic backgrounds through marker-assisted selection (MAS). Twelve breeding lines were tested for their spectra of resistance against 35 blast isolates. The results indicated that both qBSRLs were effective in new genetic backgrounds. The flanking markers and qBSRLs identified in the large mapping population showed high selection accuracy and effectiveness, suggesting the routine deployment of MAS technique in rice breeding programmes.

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Acknowledgements

This research was supported by the Rockefeller Foundation and National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand. We would like to thank Rice Gene Discovery Unit, the cooperation of BIOTEC, NSTDA and Kasetsart University, Kamphaeng Saen campus and DNA Technology Laboratory, Kasetsart University, Kamphaeng Saen campus for providing lab equipment and space for DNA and data analysis.

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Author notes

  1. Siripar Korinsak and Chanakarn Wongsaprom contributed equally to this work.

Authors and Affiliations

  1. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Rd., Khlong Luang, 12120, Pathum Thani, Thailand

    Siripar Korinsak, Chanakarn Wongsaprom, Watchareewan Jamboonsri, Saengchai Sriprakhon & Theerayut Toojinda

  2. Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Chiangmai, 50300, Thailand

    Kanyanat Sirithunya

  3. Faculty of Agriculture at Kamphaeng Saen, Department of Agronomy, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Apichart Vanavichit

Authors
  1. Siripar Korinsak
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  2. Chanakarn Wongsaprom
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  3. Watchareewan Jamboonsri
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  4. Saengchai Sriprakhon
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  5. Kanyanat Sirithunya
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  6. Apichart Vanavichit
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  7. Theerayut Toojinda
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Correspondence to Theerayut Toojinda.

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Corresponding editor: Arun Joshi

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Korinsak, S., Wongsaprom, C., Jamboonsri, W. et al. Identification of broad-spectrum resistance QTLs against rice blast fungus and their application in different rice genetic backgrounds. J Genet 101, 16 (2022). https://doi.org/10.1007/s12041-021-01357-4

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  • DOI: https://doi.org/10.1007/s12041-021-01357-4

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