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Knockout of Cia5 gene using CRISPR/Cas9 technique in Chlamydomonas reinhardtii and evaluating CO2 sequestration in control and mutant isolates

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Abstract

CRISPR/Cas9 technology is one of the common methods of genome editing and targeted gene mutation, which has recently been used for manipulating microalgae such as Chlamydomonas reinhardtii. Besides, this technology can play a role in the fight against greenhouse gases (e.g., carbon dioxide) production by studying genetic pathways to improve algal strains. Among several genes in algae that respond to CO2 and regulators control the expression of each; Cia5 is one of the most critical transcriptional regulators. In this research, we knocked out the Cia5 gene using the CRISPR/Cas9 technique and analysed the ability of C. reinhardtii to perform CO2 sequestration. Our results showed that C. reinhardtii has better performance (i.e., response to CO2 treatment) in both control and mutant species at 0.5% CO2 concentration than other concentrations. However, the difference between the control microalgae species and the mutant species was in the CO2 removal efficiency. Additionally, our findings revealed that the control type isolate in CO2 concentrations of 0.04%, 0.5% and 1% had removal efficiencies of 27%, 37% and 21%, respectively. Nevertheless, for mutant species in the same concentrations, the observed removal efficiencies were 16%, 23% and 9%.

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Acknowledgement

Authors acknowledge Dr Bagheri Bajestaani (Technical and Vocational University, Damghan, Iran) who provided C. reinhardtii used in this study.

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Authors and Affiliations

  1. Faculty of Basic Sciences, Biology Research Center, IHU, 1698715461, Tehran, Iran

    MEHRDAD ASADIAN & MOJTABA SAADATI

  2. Faculty of Agriculture, Department of Plant Breeding and Biotechnology, University of Zabol, 98615-538, Zabol, Iran

    MEHRDAD ASADIAN & NAFISEH MAHDINEZHAD

  3. Amirabad Agricultural School, Technical and Vocational University, 36716-41167, Damghan, Iran

    FARIBA BAGHERI BAJESTANI

  4. School of Biological Sciences, Monash University, Clayton, VIC, 3800, Australia

    JOHN BEARDALL

  5. Faculty of Agriculture, Department of Plant Protection, Campus of Agriculture and Natural Resources, Razi University, 6714414971, Kermanshah, Iran

    FATEME ABDOLAHADI

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  1. MEHRDAD ASADIAN
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  2. MOJTABA SAADATI
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  3. FARIBA BAGHERI BAJESTANI
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  5. FATEME ABDOLAHADI
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  6. NAFISEH MAHDINEZHAD
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Correspondence to MEHRDAD ASADIAN.

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Corresponding editor: Manoj Prasad

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ASADIAN, M., SAADATI, M., BAJESTANI, F.B. et al. Knockout of Cia5 gene using CRISPR/Cas9 technique in Chlamydomonas reinhardtii and evaluating CO2 sequestration in control and mutant isolates. J Genet 101, 6 (2022). https://doi.org/10.1007/s12041-021-01350-x

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  • DOI: https://doi.org/10.1007/s12041-021-01350-x

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