Abstract
The CP2 transcription factors are highly conserved in metazoans, where they are divided into two groups: grainyhead and late SV40 factor (LSF). We traced their evolutionary history in the Hexapoda using over 500 insect transcriptomes, to test the hypothesis that the evolution of holometaboly involved novel isoforms of these genes. All insects appear to express at least one grainyhead and one LSF-like gene, regardless of life cycle, as in most known metazoa. No major evolutionary events in these gene families occurred during the evolution of insects.
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Corresponding editor: T. N. C. Vidya
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12041_2021_1328_MOESM1_ESM.xlsx
Supplementary file1 The organisms sampled for the GRH/LSF phylogeny. Accession numbers are for the Transcriptome Shotgun Assembly file on GenBank, or the nucleotide annotation for published genomic sequences. The final column shows the number of MAFFT-aligned transcripts. A zero indicates that either all identified transcripts were too incomplete to be included in the final phylogenetic analysis, or that none were found; n.a., no code name given (XLSX 90 KB)
12041_2021_1328_MOESM2_ESM.txt
Supplementary file2 The putative GRH/LSF transcripts identified from the transcriptome mining, with the original GenBank headings (TXT 2657 KB)
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Shelomi, M., Tan, T.Z. & Huang, R.YJ. Evolution of CP2 transcription factors in Hexapoda. J Genet 100, 83 (2021). https://doi.org/10.1007/s12041-021-01328-9
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DOI: https://doi.org/10.1007/s12041-021-01328-9