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DNAH17 is essential for rat spermatogenesis and fertility

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Abstract

Knockout Dnah17 rats were generated due to its potential involvement in myopia. Subsequent study suggested that the homozygous truncation variants in DNAH17 is associated with male fertility but not myopia. Sperm count and sperm motility were measured in male rats. HE staining, immunofluorescence staining and TUNEL staining were used to observe the gross and histopathology of testis in homozygous and wild rats. Dnah17 knockout rats were generated by CRISPR/Cas9 gene editing. In the process of breeding rats, homozygous male rats were noted to be infertile, with significantly decreased number of sperm suggesting cryptozoospermia that was further confirmed by histologic studies. TUNEL assay showed an increased apoptosis in homozygous testes compared with wild type (P<0.001). A significant reduction of spermatocytes was observed in homozygotes compared with wild type (P=0.025) by immunofluorescence. These results suggest that DNAH17 is critical for spermatogenesis in male rats.

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Acknowledgements

This work was supported by grants from National Natural Science Foundation of China (81770965) and the Fundamental Research Funds of the State Key Laboratory of Ophthalmology.

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Author notes

  1. Liling Chen and Jiamin Ouyang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, People’s Republic of China

    Liling Chen, Jiamin Ouyang, Xueqing Li, Xueshan Xiao, Wenmin Sun, Shiqiang Li, Lin Zhou & Qingjiong Zhang

  2. Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China

    Yuandong Liao

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  1. Liling Chen
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  2. Jiamin Ouyang
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  5. Wenmin Sun
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Correspondence to Qingjiong Zhang.

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Corresponding editor: S. Ganesh

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Chen, L., Ouyang, J., Li, X. et al. DNAH17 is essential for rat spermatogenesis and fertility. J Genet 100, 14 (2021). https://doi.org/10.1007/s12041-021-01264-8

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  • DOI: https://doi.org/10.1007/s12041-021-01264-8

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