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Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris)

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Abstract

Lentil is one of the most important food legume species, however its genetic and genomic resources remained largely uncharacterized and unexploited. In the past few years, a number of genetic maps have been constructed and marker resources have been developed in lentil. These resources could be exploited for understanding the extent and distribution of genetic variation in genus Lens and also for developing saturated and consensus genetic maps suitable for quantitative trait loci (QTL) mapping and marker-assisted selection. The present study aims to enrich polymerase chain reaction-based linkage map of F10 recombinant inbred lines (RILs) population of 94 individuals derived from cross WA8649090 × Precoz and identification of QTLs linked to early plant vigour traits. Of the 268 polymorphic markers (93 simple sequence repeats (SSR), three inter-simple sequence repeats (ISSRs) and 172 random amplified polymorphic DNA (RAPDs)), 265 (90 SSRs, three ISSRs and 172 RAPDs) were mapped on seven linkage groups, varying in length between 25.6 and 210.3 cM, coverage of 809.4 cM with an average marker spacing of 3.05 cM. The study also reported assigning of 24 new cross-genera SSRs of Trifolium pratense on the present linkage map. The RILs along with the parents were screened for shoot length, root length, seedling length, dry weight, number of leaves and number of branches based on two replications under polyhouse conditions. A QTL-hotspot consisting of six QTLs for shoot length (cm), root length (cm) and seedling length (cm) was observed between a map distances of 56.61 and 86.81 cM on LG1.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, 176 062, India

    Rushikesh Mane, Megha Katoch, Reecha Sharma & R. K. Chahota

  2. Department of Biotechnology, ICAR-Indian Grassland and Fodder Research Institute, Jhansi, 284 003, India

    Maneet Singh

  3. ICAR-Indian Institute of Agricultural Biotechnology, Garhkhatanga, Ranchi, 834 010, India

    T. R. Sharma

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  1. Rushikesh Mane
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  2. Megha Katoch
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  4. Reecha Sharma
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  5. T. R. Sharma
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  6. R. K. Chahota
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Correspondence to R. K. Chahota.

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Corresponding editor: Manoj Prasad

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Mane, R., Katoch, M., Singh, M. et al. Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris). J Genet 99, 21 (2020). https://doi.org/10.1007/s12041-020-1182-2

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