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SNP of AHSA2 gene in three cattle breeds using snapshot technology

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Abstract

Droughtmaster is a tropical breed of beef cattle that can survive in hot climates and easily adapt to torrid environments. These traits are important in livestock breeding. In this study, we genotyped five single-nucleotide polymorphisms (SNPs) of the AHSA2 gene from 190 cattle belonging to three different breeds (Droughtmaster, Angus and Simmental) by using snapshot technology. This work aimed to identify the valuable molecular marker of heat resistance in cattle. Results showed that Droughtmaster exhibited higher expected heterozygosity and polymorphic information content compared with the two other breeds. The AHSA2-1 locus deviated from the Hardy‒Weinberg equilibrium in the Droughtmaster breed (P < 0.05). Two SNPs in Droughtmaster diverged significantly from Angus and Simmental. The SNPs were identified as AHSA2-3 and AHSA2-4, which were closely linked to the three breeds based on pair-wise FST. AHSA2-4 involved a missense mutation. In summary, the GG genotypes in AHSA2-3 and AHSA2-4 may be candidate genotypes associated with heat resistance traits and may serve as valuable genetic markers for breeding of heat-tolerant beef cattle in the future.

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Acknowledgements

This work was supported by the Characteristic Germplasm Resources Population Selection and Innovation on Mutton Sheep and Goats (no. 2015BAD03B05), National Natural Science Foundation of China (no. 31172195), Fundamental Research Funds for the Central Universities (XDJK2018B014), Chongqing Research Program of Basic Research and Frontier Technology (cstc2018jcyjAX0153), Open Project Program of State Key Laboratory of Barley and Yak Gemplasm Resources and Genetics Improvement (Tibet Academy of Agricultural and Animal Husbandry Sciences (TAAAS)), Lhasa Tibet 850002, China.

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Author notes

  1. Shu-Zhu Cheng, Cheng-Li Liu, E. Guang-Xin contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Chongqing Key Laboratory of Forage and Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, 400716, People’s Republic of China

    Shu-Zhu Cheng, E. Guang-Xin, Cheng-Li Liu, Ri-Su Na, Yan-Guo Han, Yan Zeng, Xiao Wang, Wei-Wei Ni, Bai-Gao Yang, Xing-Hai Duan, Ze-Hui Guo & Yong-Fu Huang

  2. State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement (Tibet Academy of Agricultural and Animal Husbandry Science (TAAAS)), Lhasa, 850002, Tibet, People’s Republic of China

    Wang-Dui Basang & Yan-Bin Zhu

  3. Qixia Manor Veterinary Station, Qixia, Shandong, 265300, People’s Republic of China

    Meihua Song

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  1. Shu-Zhu Cheng
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Correspondence to Yong-Fu Huang.

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Corresponding editor: H. A. Ranganath

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Cheng, SZ., Guang-Xin, E., Liu, CL. et al. SNP of AHSA2 gene in three cattle breeds using snapshot technology. J Genet 99, 54 (2020). https://doi.org/10.1007/s12041-020-01211-z

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  • DOI: https://doi.org/10.1007/s12041-020-01211-z

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