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Beadex, a homologue of the vertebrate LIM domain only protein, is a novel regulator of crystal cell development in Drosophila melanogaster

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Abstract

Haematopoiesis is a complex process in which the regulatory mechanisms of several implicated transcription factors remain uncertain. Drosophila melanogaster is an excellent model to resolve the unanswered questions about the blood cell development. This study describes the role of Beadex, a Drosophila homologue of LIM domain only 2 (LMO2), in haematopoiesis. Mutants of Beadex were analysed for blood cell abnormalities. Crystal cells, a subset of haemocytes, were significantly more in Beadex hypermorphic flies. Similarly, Beadex misexpression in prohemocytes altered the crystal cell numbers. Stage-specific misexpression analyses demonstrated that Beadex functions after the prohemocytes enter the crystal cell lineage. We also discovered that Pannier–U-shaped complex is a negative regulator of the crystal cell differentiation and is possibly negatively regulated by Beadex through its interaction with Pannier. We, therefore, suggest the mechanism of two novel regulators of crystal cell specification—Beadex and Pannier—during Drosophila haematopoiesis.

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Acknowledgments

We thank Amartya Mukherjee for help with editing. We acknowledge the Indian Institute of Science (IISc), Department of Science and Technology (DST) (DST FIST, 2008 – 2013 ref. no. SR/FST/LSII-018/2007), the University Grant Commission (UGC-SAP to MRDG: ref. no. F.3-47/2009 (SAP-II) and the Department of Biotechnology (DBT), Govt. of India, (DBT-IISC Partnership Programme for Advanced Research in Biological Sciences and Bioengineering sanction order no: DBT/BF/PRIns/2011-12/IISc/28.9.2012) for financial assistance.

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  1. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, 560 012, India

    ARUNITA CHATTERJEE, KUMAR AAVULA & UPENDRA NONGTHOMBA

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  1. ARUNITA CHATTERJEE
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  2. KUMAR AAVULA
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  3. UPENDRA NONGTHOMBA
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Correspondence to UPENDRA NONGTHOMBA.

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Corresponding editor: H. A. Ranganath

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CHATTERJEE, A., AAVULA, K. & NONGTHOMBA, U. Beadex, a homologue of the vertebrate LIM domain only protein, is a novel regulator of crystal cell development in Drosophila melanogaster. J Genet 98, 107 (2019). https://doi.org/10.1007/s12041-019-1154-6

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  • DOI: https://doi.org/10.1007/s12041-019-1154-6

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