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Journal of Genetics

, 98:14 | Cite as

Genetic diversity and population structure of two endemic Cupressus (Cupressaceae) species on the Qinghai-Tibetan plateau

  • Yaru Fu
  • Shaoke Li
  • Qiqiang Guo
  • Weilie Zheng
  • Rui Yang
  • Huie LiEmail author
Research Article
  • 104 Downloads

Abstract

Cupressus gigantea and C. torulosa are ecologically and economically important endemic species of the conifer family Cupressaceae on the Qinghai-Tibetan plateau. C. gigantea was previously classified as a subspecies of C. torulosa because of their similar morphological characteristics and close distribution. In this study, 401 individuals were sampled from 16 populations of the two Cupressus species. The specimens were genotyped using 10 polymorphic microsatellite loci through fluorescence polymerase chain reaction (PCR). The genetic diversity of C. gigantea and C. torulosa populations was generally low, with the highest genetic diversity detected in the population LLS of C. gigantea. Distance-based phylogenetic and principal co-ordinates analyses indicated a clear genetic structures for the 16 populations of the two Cupressus species. Moreover, Mantel test results showed indistinctive correlations between population-pairwise \(F_{\mathrm{st}}\) values and geographic distances, as well as between genetic distances and geographic distances in C. gigantea and C. torulosa, respectively. AMOVA suggested that genetic variation mostly resided within populations. Sixteen natural populations were evidently clustered into two major groups in the constructed neighbour-joining tree. The results demonstrated that C. gigantea and C. torulosa are different Cupressus species. The genetic information provided important theoretical references for conservation and management of the two endangered Cupressus species.

Keywords

genetic diversity genetic structure microsatellite Cupressus gigantea Cupressus torulosa 

Notes

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (31460207) and Programme of Collaborative Innovation Center Construction of Research and Development on Tibetan Characteristic Agricultural and Animal Husbandry Resources—plateau ecology, and Promotion Plan of Plateau Basic Ecological Academic Team Ability and Science and technology projects of Guizhou Province (JZ[2014]200211), China.

Supplementary material

12041_2019_1059_MOESM1_ESM.docx (4 mb)
Supplementary material 1 (docx 4105 KB)

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Yaru Fu
    • 1
    • 2
    • 3
  • Shaoke Li
    • 1
    • 2
    • 3
  • Qiqiang Guo
    • 1
    • 2
    • 3
  • Weilie Zheng
    • 1
    • 2
    • 3
  • Rui Yang
    • 4
  • Huie Li
    • 1
    • 5
    Email author
  1. 1.Tibet Agricultural and Animal Husbandry CollegeNyingchiPeople’s Republic of China
  2. 2.Key Laboratory of Forest Ecology in Tibet Plateau, Ministry of EducationNyingchiPeople’s Republic of China
  3. 3.National Key Station for Field Scientific Observation and ExperimentNyingchiPeople’s Republic of China
  4. 4.College of ForestryGuizhou UniversityGuiyangPeople’s Republic of China
  5. 5.College of AgricultureGuizhou UniversityGuiyangPeople’s Republic of China

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