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Identification of sex using SBNO1 gene in the Chinese softshell turtle, Pelodiscus sinensis (Trionychidae)

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Abstract

The Chinese softshell turtle exhibits ZZ/ZW sex determination. To identify the sex of embryos, juvenile and adult individuals, we designed two pairs of polymerase chain reaction primers, SB1-196, which amplifies a fragment of 196 bp in the female and the other, CK1-482, which amplifies the 482-bp fragment in both the sexes. It is validated in 24 adult turtles of known sex, sampled from three different locations. This one-step sexing technique is rapid and easy to perform and is reported for the first time.

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Acknowledgements

This work was supported by the National Key Programme (2016YFC0503200) from the Ministry of Science and Technology of China, a special grant from the State Forestry Administration, and the Fundamental Research Funds for the Central Universities of China. We are grateful to Xiaoshan Tianfu Bio-Technology, Hangzhou, Zhejiang Province, China, for providing samples of the Chinese softshell turtles of Japanese population.

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Author notes

  1. Lan Zhao and Xin Wang contributed equally to this work.

Authors and Affiliations

  1. The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Centre for Gene Resources of Endangered Wildlife, College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Lan Zhao, Xin Wang, Qiu-Hong Wan & Sheng-Guo Fang

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  1. Lan Zhao
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  2. Xin Wang
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  3. Qiu-Hong Wan
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  4. Sheng-Guo Fang
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Corresponding author

Correspondence to Sheng-Guo Fang.

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Corresponding editor: H. A. Ranganath

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Zhao, L., Wang, X., Wan, QH. et al. Identification of sex using SBNO1 gene in the Chinese softshell turtle, Pelodiscus sinensis (Trionychidae). J Genet 98, 36 (2019). https://doi.org/10.1007/s12041-018-1048-z

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  • DOI: https://doi.org/10.1007/s12041-018-1048-z

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