Abstract
The tiger frog Hoplobatrachus rugulosus (Wiegmann 1834) is a large robust dicroglossid frog widely distributed in southern China, Malaysia, Myanmar, Vietnam and Thailand. The escaped bred tiger frog introduced from Thailand hybridized with Chinese native population may have affected the genetic diversity of local Chinese tiger frogs. However, previous microsatellite loci of this species do not offer enough information to construct the genetic map. Here, we reported 33 new microsatellite loci from transcriptome sequencing for H. rugulosus. Alleles ranged between 1 and 10 per locus and only one locus (HRT001) was monomorphic. The polymorphic information content, observed and expected heterozygosity were 0–0.794, 0–0.969 and 0–0.831, respectively. None of the loci was observed in linkage disequilibrium and two loci (HRT023 and HRT068) deviated from Hardy–Weinberg equilibrium after Bonferroni correction for multiple tests. These transcriptome-derived microsatellite markers will be used to study the genetic divergence and construct the genetic map in H. rugulosus.
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Acknowledgements
Our experimental procedures complied with the current laws on animal welfare and research in China. This study was funded by the National Science Foundation of China (31500308), Zhejiang Provincial National Science Foundation of China (LQ16C040001), China Postdoctoral Science Foundation (2016M601843) and Key Research Projects of Lishui City (20151206, SH2017001) and Zhejiang Science and Technology Innovation Programme for College Students (2018R434003). We thank Jing-Yi Chen and Hua-Li Hu for their help during the study.
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Chen, Z., Ding, G., Wang, Y. et al. Development and characterization of 33 microsatellite loci for the tiger frog Hoplobatrachus rugulosus (Wiegmann 1834) through transcriptome sequencing. J Genet 97 (Suppl 1), 147–151 (2018). https://doi.org/10.1007/s12041-018-1029-2
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DOI: https://doi.org/10.1007/s12041-018-1029-2