Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure of Aegilops uniaristata
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Low-molecular-weight glutenin subunits (LMW-GSs) are one of the important factors for the dough processing quality. In this study, a novel LMW-GS, designated LMW-N13, from the wheat relative species Aegilops uniaristata PI 554421 was cloned and characterized. Unlike previously published LMW-GSs, LMW-N13 has a large molecular weight and is the largest LMW-GS published thus far. Sequence alignments demonstrated that LMW-N13 is a LMW-i-type subunit but contains nine cysteine residues which is one more than typical LMW-i-type subunits. In addition, four insertions are present in the repetitive domain that resulted in the large molecular weight. In vitro analysis showed that LMW-N13 could improve the dough quality of different base flours.
Keywordslow-molecular-weight glutenin subunit molecular weight processing quality Aegilops uniaristata
We thank Prof. Lihui Li for providing the Ae. uniaristata PI 554421. This work was supported by the National Natural Science Foundation of China (31471488 and 31520103911), the Key Technology Projects of Independent Innovation of Shandong Province, China (2014GJJS0201-3-1) and Shandong Agricultural Seed Improvement Project.
- Chen F. G., Zhao F., Liu R. K. and Xia G. M. 2011 Functional properties of two low-molecular-weight glutenin subunits carrying additional cysteine residues from hybrid introgression line II-12 derived from Triticum aestivum and Agropyron elongatum. Food Chem. 127, 1773–1776.Google Scholar
- Ma C. Y., Yang Y., Li X. H., Ge P., Guo G. F., Subburaj S. et al. 2013 Molecular cloning and characterization of six novel HMW-GS genes from Aegilops speltoides and Aegilops kotschyi. Plant Breed. 132, 248–239.Google Scholar
- Wang S. L., Li X. H., Wang K., Wang X. Z., Li S. S., Zhang Y. Z. et al. 2011 Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci. Genome 54, 273–284.Google Scholar