A new set of mulberry-specific SSR markers for application in cultivar identification and DUS testing
- 76 Downloads
The monophagous domesticated silkworm Bombyx mori derives all its nutritional requirement from the mulberry (Morus spp.) leaves. Therefore, mulberry has a key role in sustainably improving the quality and yield of silk. Lack of codominant markers for use in genetic mapping has constrained the efforts in marker-assisted breeding for targeted trait improvement. Currently, a total of 443 (160 genomic and 283 genic) mulberry-specific simple sequence repeat (SSR) markers are available for genome analysis (Aggarwal et al.2004; Tani et al.2005; Zhao et al.2005; Gao 2013; Mathithumilan et al.2013; Thumilan et al.2016). The draft genome sequence of Morus notabilis was made available to the mulberry research community by He et al. (2013). Krishnan et al. (2014a) mined SSRs available in the genome sequence and archived them in the MulSatDB. In this study, we report the development and validation of 24 primer pairs for the SSRs archived in MulSatDB. We have used these markers to...
Keywordsmulberry simple sequence repeats genotyping genetic analysis cultivar identification DUS testing
This study was funded by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India, New Delhi grant no. BT/PR5811/PDB/19/238/2012 sanctioned to VGN. MVP was supported with Senior Research Fellowship by the Council of Scientific and Industrial Research, New Delhi. The authors gratefully acknowledge the help received from Mr M. S. Rukmangada, CSRTI, Mysuru during genomic DNA extraction and QC. The authors also thank Prof. H. S. Prakash, Department of Studies in Biotechnology, University of Mysore, Mysuru for extending the NanoDrop 2000C facility.
- Gao L. 2013 Development of EST-SSR primers in mulberry. J. Southern Agri. 44, 1254–1257.Google Scholar
- Gray E. 1990 Evidence of phenotypic plasticity in mulberry (Morus L.). Castanea 55, 272–281.Google Scholar
- Gray E. and Call N. M. 1994 Effects of induced plant injury on leaf lobation in red mulberry (Morus rubra L.). Castanea 59, 167–175.Google Scholar
- Krishnan R. R. 2014 Development of panel of diverse germplasm and core subset of mulberry (Morus spp.) by microsatellite marker aided analysis. Ph.D. Thesis, University of Mysore, Mysuru.Google Scholar
- Mathithumilan B., Kadam N. N., Biradar J., Reddy S. H., Ankaiah M., Narayanan M. J. et al. 2013 Development and characterization of microsatellite markers for Morus spp. and assessment of their transferability to other closely related species. BMC Plant Biol. 13, 194.CrossRefPubMedPubMedCentralGoogle Scholar
- Pinto M. V., Naik V. G. and Qadri S. M. H. 2012 Genetic variability studies in mulberry using microsatellite markers. J. Sericult. Technol. 3, 38–43.Google Scholar
- Sambrook J. and Russell D. W. 2001 Molecular cloning: a laboratory manual, 3rd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar