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Oestrogen regulates the expression of cathepsin E-A-like gene through ER\(\upbeta \) in liver of chicken (Gallus gallus)

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Abstract

The cathepsin E-A-like, also known as ‘similar to nothepsin’, is a new member of the aspartic protease family, which may take part in processing of egg yolk macromolecules, due to it was identified in the chicken egg-yolk. Previously, studies have suggested that the expression of cathepsin E-A-like increased gradually during sexual maturation of pullets, but the exact regulation mechanism is poorly understood. In this study, to gain insight into the function and regulation mechanism of the gene in egg-laying hen, we cloned the cathepsin E-A-like gene and evaluated its evolutionary origin by using both phylogenetic and syntenic methods. The mode of the gene expression regulation was analysed through stimulating juvenile hens with \(17\upbeta \)-estradiol and chicken embryo hepatocytes with \(17\upbeta \)-estradiol combined with oestrogen receptor antagonists including MPP, ICI 182,780 and tamoxifen. Our results showed that cathepsin E-A-like was an orthologoues gene with nothepsin, which is present in birds but not in mammals. The expression of cathepsin E-A-like significantly increased in a dose-dependent manner after the juvenile hens were treated with \(17\upbeta \)-estradiol (\(P~<~0.05\)). Compared with the \(17\upbeta \)-estradiol treatment group, the expression of cathepsin E-A-like was not significantly changed when the hepatocytes were treated with \(17\upbeta \)-estradiol combined with MPP (\(P~<~0.05\)). In contrast, compared with the \(17\upbeta \)-estradiol combined with MPP treatment group, the expression of cathepsin E-A-like was significantly downregulated when the hepatocytes were treated with \(17\upbeta \)-estradiol combined with tamoxifen or ICI 182,780 (\(P~<~0.05\)). These results demonstrated that cathepsin E-A-like shared the same evolutionary origin with nothepsin. The expression of cathepsin E-A-like was regulated by oestrogen, and the regulative effect was predominantly mediated through ER-\(\upbeta \) in liver of chicken.

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Acknowledgements

This research was supported by the Key Science and Technology Research of Henan Province (no. 151100110800), Rolling Support Project for Innovation Research Team of Ministry of Education (no. IRT16R23) and the International Cooperation Project of Henan, China (no. 162102410030).

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Author notes

  1. Hang Zheng and Hong Li contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Hang Zheng, Hong Li, Wenbo Tan, Chunlin Xu, Lijuan Jia, Dandan Wang, Zhuanjian Li, Gunrong Sun, Xiangtao Kang, Fengbin Yan & Xiaojun Liu

  2. Henan Innovative Engineering Research Centre of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Hong Li, Zhuanjian Li, Gunrong Sun, Xiangtao Kang, Fengbin Yan & Xiaojun Liu

  3. International Joint Research Laboratory for Poultry Breeding of Henan, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Xiangtao Kang & Xiaojun Liu

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  1. Hang Zheng
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Correspondence to Xiaojun Liu.

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Corresponding editor: Silvia Garagna

ZH and LH performed the experiments, analysed the data and wrote the manuscript. TW and XC participated in the management of the experimental animals and contributed to the qPCR analyses. JL and WD participated in the sample collection, RNA isolation and contributed to the qPCR analyses. LZ and SG participated in the management of the experimental animals. KX and YF participated in the experiment design and discussion. LX conceived the study and wrote the manuscript. All authors have read and approved the final manuscript.

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Zheng, H., Li, H., Tan, W. et al. Oestrogen regulates the expression of cathepsin E-A-like gene through ER\(\upbeta \) in liver of chicken (Gallus gallus). J Genet 97, 145–155 (2018). https://doi.org/10.1007/s12041-018-0890-3

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  • DOI: https://doi.org/10.1007/s12041-018-0890-3

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