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Genomewide analysis of MATE-type gene family in maize reveals microsynteny and their expression patterns under aluminum treatment

Journal of Genetics volume 95, pages 691–704 (2016)Cite this article

Abstract

Multidrug and toxic compound extrusion (MATE) proteins are a group of secondary active transporters, which widely exist in all living organisms and play important role in the detoxication of endogenous secondary metabolites and exogenous agents. However, to date, no systematic and comprehensive study of this family is reported in maize. Here, a total of 49 MATE genes (ZmMATE) were identified and divided into seven groups by phylogenetic analysis. Conserved intro–exon structures and motif compositions were investigated in these genes. Results by gene locations indicated that these genes were unevenly distributed among all 10 chromosomes. Tandem and segmental duplications appeared to contribute to the expansion and evolution of this gene family. The K a/ K s ratios suggested that the ZmMATE has undergone large-scale purifying selection on the maize genome. Interspecies microsynteny analysis revealed that there were independent gene duplication events of 10 ZmMATE. In addition, most maize MATE genes exhibited different expression profiles in diverse tissues and developmental stages. Sixteen MATE genes were chosen for further quantitative real-time polymerase chain reaction analysis showed differential expression patterns in response to aluminum treatment. These results provide a useful clue for future studies on the identification of MATE genes and functional analysis of MATE proteins in maize.

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Acknowledgements

This study was supported by the key project of National Natural Science Foundation of China (31501321) the Anhui University Natural Science Research Projects (KJ2015A100) and Anhui Postdoctoral Science Foundation and Colleges. We would like to thank the members of the Key Laboratory of Crop Biology of Anhui province for their assistance in this study.

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    1. Key Laboratory of Crop Biology of Anhui Province, Anhui Agricultural University, Hefei, 230036, China

      HUASHENG ZHU, JIANDONG WU, YINGLI JIANG, JING JIN, WEI ZHOU, YU WANG, GUOMIN HAN, YANG ZHAO & BEIJIU CHENG

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    1. HUASHENG ZHU
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    Correspondence to BEIJIU CHENG.

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    [Zhu H., Wu J., Jiang Y., Jin J., Zhou W., Wang Y., Han G., Zhao Y. and Cheng B. 2016 Genomewide analysis of MATE-type gene family in maize reveals microsynteny and their expression patterns under aluminum treatment. J. Genet. 95, xx–xx]

    Huasheng Zhu and Jiandong Wu contributed equally to this work.

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    ZHU, H., WU, J., JIANG, Y. et al. Genomewide analysis of MATE-type gene family in maize reveals microsynteny and their expression patterns under aluminum treatment. J Genet 95, 691–704 (2016). https://doi.org/10.1007/s12041-016-0686-2

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    • DOI: https://doi.org/10.1007/s12041-016-0686-2

    Keywords

    • multidrug and toxic compound extrusion
    • gene family
    • duplicated gene
    • microsynteny
    • expression profiles
    • maize.