Comparative cytogenetic analysis of four species of Dendropsophus (Hylinae) from the Brazilian Atlantic forest
We conducted a cytogenetic study of four hyline frog species (Dendropsophus elegans, D. microps, D. minutus and D. werneri) from southern Brazil. All species had 2n = 30 chromosomes, with interspecific and intraspecific variation in the numbers of metacentric, submetacentric, subtelocentric and telocentric chromosomes. C-banding and fluorochrome staining revealed conservative GC-rich heterochromatin localized in the pericentromeric regions of all species. The location of the nucleolus organizer regions, as confirmed by fluorescent in situ hybridization, differed between species. Telomeric probes detected sites that were restricted to the terminal regions of all chromosomes and no interstitial or centromeric signals were observed. Our study corroborates the generic synapomorphy of 2n = 30 chromosomes for Dendropsophus and adds data that may become useful for future taxonomic revisions and a broader understanding of chromosomal evolution among hylids.
KeywordsAnura C-banding nucleolus organizer regions telomeres Dendropsophus.
We would like to express our thanks to two anonymous reviewers for helpful comments that improved the manuscript; Michele Orane, Luiz Fernando Kraft, Rafael Balen and Gisele Perazzo for help in lab work; the Grassmann family for permision to collect on their property; and Instituto Chico Mendes de Conservação da Biodiversidade, Instituto Ambiental do Paraná for environmental licenses. Besides, we are very grateful to Dr Mark A. McPeek for revising the English style of the manuscript. This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico eTecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo.
- Amaro-Ghilardi R. C., Silva M. J. J., Rodrigues M. T. and Yonenaga-Yassuda Y. 2008 Chromosomal studies in four species of genus Chaunus (Bufonidae, Anura): localization of telomeric and ribosomal sequences after fluorescence in situ hybridization (FISH). Genetica 134, 159–168.CrossRefPubMedGoogle Scholar
- Badissera Jr F. A., Oliveira P. S. L. and Kasahara S. 1993 Cytogenetics of four Brazilian Hyla species (Amphibia, Anura) and description of a case of supernumerary chromosome. Rev. Brasil Gen. 16, 335–345.Google Scholar
- Bogart J. 1973 Evolution of anuran karyotypes. In: Evolutionary biology of Anurans (ed. J. Vial). University of Missouri Press, Columbia.Google Scholar
- Fouquet A., Noona B. P., Blanc M. and Orrico V. G. D. 2011 New distributional records and phylogenetic position for Dendropsophus gaucheri. Zootaxa 3035, 59–67.Google Scholar
- Frost D. R. 2014 Amphibian species of the world: an online reference, version 6.0 (accessed on 13th March 2014). Electronic database accessible at http://research.amnh.org/herpetology/amphibia/index.html.
- Green D. M. and Sessions S. K. 1991 Nomenclature for chromosomes. In Amphibian cytogenetics and evolution (ed. D. M. Green and S. K. Sessions). Academic Press, San Diego.Google Scholar
- Gruber S. L., Haddad C. F. B. and Kasahara S. 2005 Evaluating the karyotypic diversity in species of Hyla (Anura; Hylidae) with 2n = 30 chromosomes based on the analysis of ten species. Folia Biol. 51, 68–75.Google Scholar
- IUCN 2013 The IUCN red list of threatened species, version 2013.2 (accessed on 2nd February 2013). Electronic database accessible at http://www.iucnredlist.org.
- Kaiser H., Mais C., Bolaños F., Steinlein C., Feichtinger W. and Schmid M. 1996 Chromosomal investigation of three Costa Rican frogs from the 30-chromosome radiation of Hyla with the description of a unique geographic variation in nucleolus organizer regions. Genetica 98, 95–102.CrossRefGoogle Scholar
- Kellogg R. 1932 Mexican tailless amphibians in the United States National Museum. Bull. U. S. Natl. Mus. 160, 1–224.Google Scholar
- Medeiros L. R., Lourenço L. B., Rossa-Feres D. C., Lima A. P., Andrade G. V., Giaretta A. et al. 2013 Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences. BMC Genet. 14, 59–76.CrossRefPubMedPubMedCentralGoogle Scholar
- Skuk G. and Langone J. A. 1992 Los cromosomas de cuatroespeciesdel género Hyla (Anura: Hylidae) com número diploide de 2n = 30. Acta Zool. Lilloana 41, 165–171.Google Scholar