Comparative cytogenetic analysis of four species of Dendropsophus (Hylinae) from the Brazilian Atlantic forest
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We conducted a cytogenetic study of four hyline frog species (Dendropsophus elegans, D. microps, D. minutus and D. werneri) from southern Brazil. All species had 2n = 30 chromosomes, with interspecific and intraspecific variation in the numbers of metacentric, submetacentric, subtelocentric and telocentric chromosomes. C-banding and fluorochrome staining revealed conservative GC-rich heterochromatin localized in the pericentromeric regions of all species. The location of the nucleolus organizer regions, as confirmed by fluorescent in situ hybridization, differed between species. Telomeric probes detected sites that were restricted to the terminal regions of all chromosomes and no interstitial or centromeric signals were observed. Our study corroborates the generic synapomorphy of 2n = 30 chromosomes for Dendropsophus and adds data that may become useful for future taxonomic revisions and a broader understanding of chromosomal evolution among hylids.
KeywordsAnura C-banding nucleolus organizer regions telomeres Dendropsophus.
We would like to express our thanks to two anonymous reviewers for helpful comments that improved the manuscript; Michele Orane, Luiz Fernando Kraft, Rafael Balen and Gisele Perazzo for help in lab work; the Grassmann family for permision to collect on their property; and Instituto Chico Mendes de Conservação da Biodiversidade, Instituto Ambiental do Paraná for environmental licenses. Besides, we are very grateful to Dr Mark A. McPeek for revising the English style of the manuscript. This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico eTecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo.
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