Skip to main content
Log in

Development and characterization of eighty-one microsatellite markers in Indian white shrimp, Fenneropenaeus indicus, through cross-amplification

  • Online Resources
  • Published:
Journal of Genetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.


  • Belkhir K., Borsa P., Goudet J., Chikhi L. and Bonhomme F. 1997 GENETIX version 4.02, Genetics logiciel sous windows pour Ia ge ne Tique des populations, available at:

  • Bezault E., Rognon X., Gharbi K., Baroiller J. F. and Chevassus B. 2012 Microsatellites cross-species amplification across some African cichlids. Int. J. Evol. Biol. 2012 (doi: 10.1155/2012/80935).

  • Chauhan T., Lal K. K., Mohindra V., Singh R. K., Punia P., Gopalakrishnan A. et al. 2007 Evaluating genetic differentiation in wild populations of the Indian major carp, Cirrhinus mrigala (Hamilton–Buchanan, 1882): evidence from allozyme and microsatellite markers. Aquaculture 269, 135–149.

  • Chen F., Zeng L. and Cheng Q. 2012 Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes (Clupeiformes: Engraulidae) and cross-species amplification in two closely related taxa. J. Genet. 91, e37–e43.

  • Cordeiro G. M., Casu R. and McIntyre C. L. 2001 Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to Eranthus and Sorghum. Plant Sci. 160, 1115–1123.

  • Dong S., Kong J., Zhang T., Meng X. and Wang R. 2006 Parentage determination of Chinese shrimp Fenneropenaeus chinensis based on microsatellite DNA markers. Aquaculture 258, 283–288.

  • Freitas P. D., Jesus C. M. and Galetti P. M. 2007 Isolation and characterization of new microsatellite loci in the Pacific white shrimp Litopenaeus vannamei and cross-species amplification in other penaeid species. Mol. Ecol. Notes 7, 324–326.

  • Gao H., Kong J., Yan B., Yu F., Luan S. and Cai S. 2008 Permanent genetic resources: twelve new microsatellite markers for the Chinese shrimp Fenneropenaeus chinensis. Mol. Ecol. Resour. 8, 325–327.

  • Gopalakrishnan A., Musammilu K. K., Muneer P. M. A., Lal K. K., Kapoor D., Ponniah A. G. et al. 2004 Microsatellite DNA markers to assess population structure of red tailed barb, Gonoproktopterus curmuca. Acta Zool. Sin. 50, 686–690.

  • Gopalakrishnan A., Muneer P. M. A., Musammilu K. K., Lal K. K., Kapoor D. and Mohindra V. 2006 Primers from the orders Osteoglossiform and Siluriform detect polymorphic microsatellite loci in sun-catfish, Horabagrus brachysoma (Teleostei: Bagridae). J. Appl. Ichthyol. 22, 456–458.

  • Guo X. Z., Zhang G. R., Wei K. J., Guo S. S., Qin J. H. and Gardner J. P. 2012 Isolation and characterization of twenty-one polymorphic microsatellite loci from Schizothorax o’connori and cross-species amplification. J. Genet. 92, e60–e64.

  • Huang W., Liang X. F., Qu C. M., Li J. and Cao L. 2012 Isolation and characterization of twenty-five polymorphic microsatellite markers in Siniperca scherzeri Steindachner and cross-species amplification. J. Genet. 91, e113–e117.

  • Jones J. W., Culver M., David V., Struthers J., Johnson N. A., Neves R. J. et al. 2004 Development and characterization of microsatellite loci in the endangered oyster mussel Epioblasma capsaeformis (Bivalvia: Unionidae). Mol. Ecol. Notes 4, 649–652.

  • Kathirvelpandian A., Gopalakrishnan A., Lakra W. S., Krishna G., Sharma R., Musammilu K. K. et al. 2014 Microsatellite markers to determine population genetic structure in the golden anchovy, Coilia dussumieri. Biochem. Genet. 52, 296–309.

  • Miller S. A., Dykes D. D. and Polesky H. F. 1988 A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16, 1215.

  • Mohitha C., Joy L., Divya P. R., Gopalakrishnan A., Basheer V. S., Koya M. et al. 2014 Characterization of microsatellite markers in Silver pomfret, Pampus argenteus (Euphrasen, 1788) (Perciformes:Stromateidae) through cross species amplification and population genetic applications. J. Genet. 93, e89– e93.

  • Moore S. S., Whan V., Davis G., Byrne K., Hetzel D. J. S. and Preston N. 1999 The development and application of genetic markers for the Kuruma prawn, Penaeus japonicus and their use in parentage determination and linkage mapping. Aquaculture 173, 19–32.

  • Rousset F. 2008 Genepop’007: a complete reimplementation of the genepop software for Windows and Linux. Mol. Ecol. Resour. 8, 103–106.

  • Van Oosterhout C., Hutchinson W. F., Wills D. P. M. and Shipley P. 2004 Micro-checker: software for identifying and correctinggenotyping errors in microsatellite data. Mol. Ecol. Notes 4, 535–538.

  • Van Oosterhout C., Weetman D. and Hutchinson W. F. 2006 Estimation and adjustment of microsatellite null alleles in nonequilibrium populations. Mol. Ecol. Notes 6, 255–256.

  • Wang Hongxia., Fuhua Li and Jianhai Xiang 2005 Polymorphic EST–SSR markers and their mode of inheritance in Fenneropenaeus chinensis. Aquaculture 249, 107–114.

  • Weber J. L. 1990 Informativeness of human (dC–dA)n.(dG–dT)n polymorphisms. Genomics 7, 524–530.

  • Xu Z., Dhar A. K., Wyrzykowski J. and Alcivar Warren A. 1999 Identification of abundant and informative microsatellites from shrimp (Penaeus monodon) genome. Anim. Genet. 30, 150–156.

  • Zardoya R., Vollmer D. M., Craddock C., Streelman J. T., Karl S. and Meyer A. 1996 Evolutionary conservation of microsatellite flanking regions and their use in resolving the phylogeny of cichlid fishes (Pisces: Perciformes). Proc. R. Soc. London, B Ser. 263, 1589–1598.

  • Zhi-Ying J. I. A., Xiao-Wen S. U. N., Li-Qun L. I. A. N. G., Da-Yu L. I. and Qing-Quan L. E. I. 2006 Isolation and characterization of microsatellite markers from Pacific white shrimp (Litopenaeus vannamei). Mol. Ecol. Notes 6, 1282–1284.

Download references


This work was funded by the Department of Biotechnology (DBT), Govt. of India (project sanction order no. BT/PR5772/AAQ/ 03/241/2005) and DNA sequencing facility was provided by Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India. We are grateful to all the Scientists and research scholars of NBFGR Cochin Centre, especially Dr P. R. Divya, Dr T. Raja Swaminathan, Dr A. Kathirvelpandian, Ms C. Mohitha and Ms K. B. Sheeba for their help and support; and Dr P. Manoj, RGCB for his support during DNA sequencing.

Author information

Authors and Affiliations


Corresponding author

Correspondence to K. A. SAJEELA.

Additional information

[Sajeela K. A., Gopalakrishnan A., Basheer V. S., Bineesh K. K. and Jena J. K. 2015 Development and characterization of eighty-one microsatellite markers in Indian white shrimp, Fenneropenaeus indicus, through cross-amplification. J. Genet. 94, e43–e50. Online only:]

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

SAJEELA, K.A., GOPALAKRISHNAN, A., BASHEER, V.S. et al. Development and characterization of eighty-one microsatellite markers in Indian white shrimp, Fenneropenaeus indicus, through cross-amplification. J Genet 94 (Suppl 2), 43–50 (2015).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: