Abstract
Functional markers (FMs) developed from sequence polymorphisms are present in allelic variants of a functional gene at a locus and are directly associated with phenotypic variations. In this study, FM linked to Rht-B1, Rht-D1, TaCwi-A1, TaSus2-2B, TaGW2-6A and Dreb-B1 genes conferring to yield potential and water-saving were selected to analyse the distribution in 102 wheat varieties, most of which were authorized in the past decade and adapted to grow in Zone II of China. First, the semi-dwarfing genes Rht-B1b and Rht-D1b (mutant alleles) conferring to grain yield were analysed. The frequencies of favourable alleles Rht-B1b and Rht-D1b were 32.4 and 58.8%, respectively. Comparing with the previous report, the frequency of Rht-B1b among cultivars in this study is similar to the frequency among cultivars released in the 1990s, while the frequency of Rht-D1b is slightly lower than the previous report 63.9%. Twelve (11.8%) cultivars neither contained Rht-B1b nor Rht-D1b, while only Yumai 66 contained both semidwarfing genes. Linyuan8 and Xinong 928 are heterozygous at RhtB1 locus and Zhengmai 9023 is heterozygous at both RhtB1 and Rht-D1 loci. Second, the TaCwi-A1, TaSus2-2B and TaGW2-6A genes considered as candidate genes related to grain weight were detected. We found that the frequencies of the favourable alleles were 76.5, 56.9 and 69.6%, respectively. Among the 102 wheat varieties, 30 contained all the three favourable genes, 45 contained two of the three favourable genes and 27 contained only one. There are eight wheat varieties (7.8%) in hybrid state at the TaCWI-A1 locus. Third, the designed FM linked to water-saving gene Dreb-B1 were validated on 102 wheat varieties. The results showed that the haplotypes of 47 wheat varieties at the Dreb-B1 locus were same as that of Opata 85, and 55 wheat varieties showed the signal expected for W7984 (Opata 85 and W7984 are parents of the ITMI mapping population). This information will be useful for the wheat breeding programmes aiming at improving yield and water use efficiency in Shijiazhuang located in China Zone II.
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References
Agarwal P. K., Agarwal P., Reddy M. K. and Sopory S. K. 2006 Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Rep. 25, 1263–1274.
Bagge M., Xia X. and Lubberstedt T. 2007 Functional markers in wheat. Curr. Opin. Plant Biol. 10, 211–216.
Chen F., Gao M., Zhang J., Zuo A., Shang X. and Cui D. 2013 Molecular characterization of vernalization and response genes in bread wheat from the Yellow and Huai Valley of China. BMC Plant Biol. 13, 199.
Ellis H., Spielmeyer W., Gale R., Rebetzke J. and Richards A. 2002 “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor. Appl. Genet. 105, 1038–1042.
He X., He Z., Zhang L., Sun D., Morris C. F., Fuerst E. P., et al. 2007 Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor. Appl. Genet. 115, 47–58.
Hu Y., Moiwo J. P., Yang Y., Han S. and Yang Y. 2010 Agricultural water-saving and sustainable groundwater management in Shijiazhuang Irrigation District, North China Plain. J. Hydrol. 219–232.
Jiang Q., Hou J., Hao C., Wang L., Ge H., Dong Y., et al. 2011 The wheat (T. aestivum) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits. Funct. Integr. Genomics 11, 49–61.
Jin X. J., Sun D. F., Li H. Y., Yang Y. and Sun G. L. 2013 Characterization and molecular mapping of a dwarf mutant in wheat. Genet. Mol. Res. 12, 3555–3565.
Khush G. S. 2001 Green revolution: the way forward. Nat. Rev. Genet. 2, 815–822.
Li A., Yang W., Lou X., Liu D., Sun J., Guo X., et al. 2013 Novel natural allelic variations at the Rht-1 loci in wheat. J. Integr. Plant Biol. 55, 1026–1037.
Liang D., Tang J., Peña R. J., Singh R., He X., Shen X., et al. 2010 Characterization of CIMMYT bread wheats for high- and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers. Euphytica 172, 235–250.
Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K., et al. 1998 Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10, 1391–1406.
Liu Y., He Z., Appels R. and Xia X. 2012 Functional markers in wheat: current status and future prospects. Theor. Appl. Genet. 125, 1–10.
Liu S., Wang X., Wang H., Xin H., Yang X., Yan J., et al. 2013 Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L. PLoS Genet. 9, e1003790.
Lyu L., Hu Y., Li Y. and Wang P. 2007 Effect of irrigation treatment on water use efficiency and yield of different wheat cultivar. J. Triticeae Crops 27, 88–92.
Ma D., Yan J., He Z., Wu L. and Xia X. 2012 Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers. Mol. Breed. 29, 43–52.
Moiwo J. P., Yang Y., Li H., Han S. and Yang Y. 2010 Impact of water resource exploitation on the hydrology and water storage in Baiyangdian Lake. Hydrol. Process. 24, 3026–3039.
Rebetzke G. J., Appels R., Morrison A. D., Richards R. A., McDonald G., Ellis M. H., et al. 2001 Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.) Aust. J. Agric. Res. 52, 1221– 1234.
Sazegari S. and Niazi A. 2012 Isolation and molecular characterization of wheat (Triticum aestivum) dehydration Responsive Element Binding Factor (DREB) isoforms. Aus. J. Crop Sci. 6, 1037–1044.
Shen Y. G., Zhang W. K., He S. J., Zhang J. S., Liu Q. and Chen S. Y. 2003 An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress. Theor. Appl. Genet. 106, 923–930.
Srinivasachary, Gosman N., Steed A., Simmonds J., Leverington-Waite M., Wang Y., et al. 2008 Susceptibility to Fusarium head blight is associated with the Rht-D1b semi-dwarfing allele in wheat. Theor. Appl. Genet. 116, 1145–1153.
Srinivasachary, Gosman N., Steed A., Hollins T. W., Bayles R., Jennings P., et al. 2009 Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight. Theor. Appl. Genet. 118, 695– 702.
Su Z., Hao C., Wang L., Dong Y. and Zhang X. 2011 Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.) Theor. Appl. Genet. 122, 211–223.
Sun D., He Z., Xia X., Zhang L., Morris C. F., Appels R., et al. 2005 A novel STS marker for polyphenol oxidase activity in bread wheat. Mol. Breed. 16, 209–218.
Wei B., Jiang R., C. W., Chen J., Mao X., Chang X., et al. 2009 Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs. Mol. Breed. 23, 13–22.
Whitford R., Fleury D., Reif J. C., Garcia M., Okada T., Korzun V., et al. 2013 Hybrid breeding in wheat: technologies to improve hybrid wheat seed production. J. Exp. Bot. 64, 5411–5428.
Wu L., Xia X., Zhu H., Li S., Zheng Y. and He Z. 2010 Molecular characterization of Lr34/Yr18/Pm38 in 273 CIMMYT wheat cultivars and lines using functional markers. Sci. Agric. Sin. 43, 4553–4561.
Yang F., He X., He Z., Shang X., Yang W. and Xia X. 2008 Molecular detection and distribution of allelic variations of a gene for yellow pigment content in Chinese winter wheat cultivars. Sci. Agric. Sin. 41, 2923–2930.
Yang F., Zhang X., Xia X., Laurie D. A., Yang W. and He Z. 2009 Distribution of the photoperiod insensitive Ppd-D1a allele in Chinese wheat cultivars. Euphytica 165, 445–452.
Zhang X., Yang S., Zhou Y., He Z. and Xia X. 2006 Distribution of the Rht-B1b, Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers. Euphytica 152, 109–116.
Zhang X., Xiao Y., Zhang Y., Xia X., Dubcovsky J. and He Z. 2008 Allelic variation at the vernalization genes Vrn-A1, Vrn-B1, Vrn-D1 and Vrn-B3 in Chinese wheat cultivars and their association with growth habit. Crop Sci. 48, 458–470.
Zhang Y. J., Fan P. S., Zhang X., Chen C. J. and Zhou M. G. 2009 Quantification of Fusarium graminearum in harvested grain by real-time polymerase chain reaction to assess efficacies of fungicides on fusarium head blight, deoxynivalenol contamination and yield of winter wheat. Phytopathology 99, 95–100.
Zhang B., Shi W., Li W., Chang X. and Jing R. 2013 Efficacy of pyramiding elite alleles for dynamic development of plant height in common wheat. Mol. Breed. 32, 327–338.
Acknowledgements
The authors are grateful to Xin Li and Dongcheng Liu of Institute of Genetics and Developmental biology, Chinese Academy of Sciences for kindly reviewing this manuscript. Financial support was provided by National 863 Program (2012AA101105) and National Key Technology Support Program (2011BAD35B03).
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Gao Z., Shi Z., Zhang A. and Guo J. 2015 Distribution of genes associated with yield potenial and water-saving in Chinese Zone II wheat detected by developed functional markers. J. Genet. 94, xx–xx
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GAO, Z., SHI, Z., ZHANG, A. et al. Distribution of genes associated with yield potential and water-saving in Chinese Zone II wheat detected by developed functional markers. J Genet 94, 35–42 (2015). https://doi.org/10.1007/s12041-015-0472-6
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DOI: https://doi.org/10.1007/s12041-015-0472-6