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Diversity of five novel Y-STR loci and their application in studies of north Chinese populations

Journal of Genetics volume 89, pages 29–36 (2010)Cite this article

Abstract

Y-chromosomal short tandem repeats (Y-STRs) show sufficient variability among individuals in a population and high degree of geographical differentiation, so their polymorphic character makes them especially suited for population genetic studies. In this study, five novel Y-STR loci were analysed in 174 samples from five minority populations residing in north China (Daur, Kazak, Xibe, Uighur and Kirgiz) to determine the diversity of these loci in north China and to evaluate their usefulness in population study. Ninety-seven haplotypes were constructed, with 30 in Daur, 24 in Kazak, 28 in Uighur, 27 in Xibe and 16 in Kirgiz. Sixty-six (68.04%) of them were unique. The R ST showed that there was no significant difference in Daur and Xibe (R ST = 0.02231, P > 0.05), while among the Kazak, Uighur and Kirgiz, who reside in northwest China, there were significant differences. These results showed that these five Y-STR loci were polymorphic in the five populations. The results of AMOVA showed that majority of the differences were found within populations. By R ST, the relationships of the five populations were accordance with the historical records: Xibe migrated to Xinjiang during the Qing Dynasty, and Kazak, Uighur and Kirgiz have different ancestors.

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References

  • Chomezynski P. and Sacchi N. 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.

    Google Scholar 

  • de Knijff P., Kayser M., Caglia A., Corach D., Fretwell N., Gehrig C. et al. 1997 Chromosome Y microsatellites: population genetic and evolutionary aspects. Int. J. Legal Med. 110, 134–149.

    Article  PubMed  Google Scholar 

  • Diederiche M., Martin P., Amorim A., Corte-Real F. and Gusmao L. 2005 A case of double alleles at three Y-STR loci: forensic implications. Int. J. Legal Med. 119, 223–225.

    Article  CAS  PubMed  Google Scholar 

  • Hall A. and Ballantyne J. 2003 The development of an 18-locus YSTR system for forensic casework. Anal. Bioanal. Chem. 376, 1234–1246.

    Article  CAS  PubMed  Google Scholar 

  • Hanson E. K. and Ballantyne J. 2004 A highly discriminating 21 locus Y-STR “megaplex” system designed to augment the minimal haplotype loci for forensic casework. J. Forensic Sci. 49, 40–51.

    Article  CAS  PubMed  Google Scholar 

  • Huang T. Y., Hsu Y. T., Li J. M., Chung J. H. and Shun C. T. 2008 Polymorphism of 17 Y-STR loci in Taiwan population. Forensic Sci. Int. 174, 249–254.

    Article  CAS  PubMed  Google Scholar 

  • Jobling M. A. and Tyler-Smith C. 1995 Fathers and sons: the Y chromosome and human evolution. Trends Genet. 11, 449–456.

    Article  CAS  PubMed  Google Scholar 

  • Jobling M. A., Pandya A. and Tyler-Smith C. 1997 The Y chromosome in forensic analysis and paternity testing. Int. J. Legal Med. 110, 118–124.

    Article  CAS  PubMed  Google Scholar 

  • Kayser M., Brauer S., Willuweit S., Schadlich H., Batzer M. A., Zawacki J. et al. 2002 Online Y-chromosomal short tandem repeat haplotype reference database (YHRD) for U.S. populations. J. Forensic Sci. 47, 513–519.

    PubMed  Google Scholar 

  • Kayser M., Kittler R., Erler A., Hedman M., Lee A. C., Mohyuddin A. et al. 2004 A comprehensive survey of human Y-chromosomal microsatellites. Am. J. Hum. Genet. 74, 1183–1197.

    Article  CAS  PubMed  Google Scholar 

  • Khodjet El Khil H., Triki Marrakchi R., Yacoubi Loueslati B., Langaney A., Fellous M. and Benammar Elgaaied A. 2001 Y chromosome microsatellite variation in three populations of Jerba Island (Tunisia). Ann. Hum. Genet. 65, 263–270.

    Article  CAS  PubMed  Google Scholar 

  • Kumagai R., Kumagai A., Saigusa K. and Aoki Y. 2007 Haplotype analysis of 17 Y-STR loci in a Japanese population. Forensic Sci. Int. 172, 72–78.

    Article  CAS  PubMed  Google Scholar 

  • Lessig R., Willuweit S., Krawczak M., Wu F. C., Pu C. E., Kim W. et al. 2003 Asian online Y-STR Haplotype reference database. Leg. Med. (Tokyo) 5,suppl 1, S160–S163.

    CAS  Google Scholar 

  • Lim S. K., Xue Y., Parkin E. J. and Tyler-Smith C. 2007 Variation of 52 new Y-STR loci in the Y chromosome consortium worldwide panel of 76 diverse individuals. Int. J. Legal Med. 121, 124–127.

    Article  PubMed  Google Scholar 

  • Mertens G., Jehaes E., Leijnen G., Rand S., Jacobs W. and Van Marck E. 2007 Twelve-locus Y-STR haplotypes in the Flemish population. J. Forensic Sci. 52, 755–757.

    Article  PubMed  Google Scholar 

  • Nei M. 1996 Phylogenetic analysis in molecular evolutionary genetics. Annu. Rev. Genet. 30, 371–403.

    Article  CAS  PubMed  Google Scholar 

  • Parkin E. J., Kraayenbrink T., van Driem G. L., Tshering O., Gaselo K., de Knijff P. and Jobling M. A. 2006 26-Locus Y-STR typing in a Bhutanese population sample. Forensic Sci. Int. 161, 1–7.

    Article  CAS  PubMed  Google Scholar 

  • Roewer L., Krawczak M., Willuweit S., Nagy M., Alves C., Amorim A. et al. 2001 Online reference database of European Y-chromosomal short tandem repeat (STR) haplotypes. Forensic Sci. Int. 118, 106–113.

    Article  CAS  PubMed  Google Scholar 

  • Ruofu D. 1994 Ethnic groups in China. Science Publishing House, Beijing, People’s Republic of China.

    Google Scholar 

  • Schoske R., Vallone P. M., Kline M. C., Redman J. W. and Butler J. M. 2004 High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays. Forensic Sci. Int. 139, 107–121.

    Article  CAS  PubMed  Google Scholar 

  • Schultes T., Hummel S. and Herrmann B. 1999 Amplification of Ychromosomal STRs from ancient skeletal material. Hum. Genet. 104, 164–166.

    Article  CAS  PubMed  Google Scholar 

  • Willuweit S. and Roewer L. 2007 Y chromosome haplotype reference database (YHRD): Update. Forensic Sci. Int. Genet. 1, 83–87.

    Article  PubMed  Google Scholar 

  • Xiao Chunjie D. R. 2000 The principal component analysis of allele frequency in China populations. Science in China (Series C) 30, 434–442.

    Google Scholar 

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Affiliations

  1. Laboratory of Medical Genetics, Harbin Medical University, Harbin, 150081, People’s Republic of China

    Zhaoyang Xu, Haiming Sun, Yang Yu, Yan Jin, Xaingning Meng, Donglin Sun, Jing Bai, Feng Chen & Songbin Fu

  2. Bio-Pharmaceutical Key Laboratory of Heilongjiang Province, Harbin, 150081, People’s Republic of China

    Songbin Fu

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  1. Zhaoyang Xu
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  2. Haiming Sun
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  3. Yang Yu
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  4. Yan Jin
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  5. Xaingning Meng
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  6. Donglin Sun
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  7. Jing Bai
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  8. Feng Chen
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  9. Songbin Fu
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Correspondence to Songbin Fu.

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Xu, Z., Sun, H., Yu, Y. et al. Diversity of five novel Y-STR loci and their application in studies of north Chinese populations. J Genet 89, 29–36 (2010). https://doi.org/10.1007/s12041-010-0008-z

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  • DOI: https://doi.org/10.1007/s12041-010-0008-z

Keywords

  • human genetics
  • Y chromosome
  • short tandem repeats (STRs)
  • north China