Journal of Genetics

, Volume 88, Issue 3, pp 315–320 | Cite as

Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker

  • Mamta Singh
  • Ravindra Kumar
  • N. S. Nagpure
  • Basdeo Kushwaha
  • Indra Mani
  • U. K. Chauhan
  • W. S. Lakra
Research Article


Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora.


18S rDNA 5S rDNA FISH rDNA Tor putitora 


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  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W. et al. 1997 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.CrossRefPubMedGoogle Scholar
  2. Bertollo L. A. C., Takahashi C. S. and Moreira-Filho O. 1978 Cytotaxonomic consideration on Hoplias lacrdae (Pisces, Erythrinidae). Braz. J. Genet. 1, 103–120.Google Scholar
  3. Campo D., Machado-Schiaffino G., Horreo J. L. and Garcia-Vazquez E. 2009 Molecular organization and evolution of 5S rDNA in the genus Merluccius and their phylogenetic implications. J. Mol. Evol. 68, 208–216.CrossRefPubMedGoogle Scholar
  4. Castro J., Santiago R., Padro B. G., Sanchez L. and Martinez P. 2001 Population analysis of an unusual NOR-site polymorphism in Brown trout (Salmo trutta L.). Heredity 86, 291–302.CrossRefPubMedGoogle Scholar
  5. Charlesworth B., Sniegowski P. and Stephan W. 1994 The evolutionary dynamics of repetitive DNA in eukaryotes. Nature 371, 215–220.CrossRefPubMedGoogle Scholar
  6. Dover G. A. 1982 Molecular drive: a cohesive model of species evolution. Nature 299, 111–117.CrossRefPubMedGoogle Scholar
  7. Drouin G. and Moniz de Sa M. 1995 The concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families. Mol. Biol. Evol. 12, 481–493.PubMedGoogle Scholar
  8. Ferro D. A. M., Neo D. M., Moreira-Filho O. and Bertollo L. A. C. 2001 Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): population distribution and functional diversity. Genetica 110, 55–62.CrossRefGoogle Scholar
  9. Fontana F., Lanfredi M., Congiu L., Leis M., Chicca M. and Rossi R. 2003 Chromosomal mapping of 18S–28S and 5S rRNA genes by two-colour fluorescent in situ hybridization in six sturgeon species. Genome 46, 473–477.CrossRefPubMedGoogle Scholar
  10. Fujiwara A., Abe S., Yamaha E., Yamazaki F. and Yoshida M. C. 1998 Chromosomal localization and hetrochromatin association of ribosomal RNA genes loci and silver stained nucleolar organizer regions in salmonid fishes. Chromosome Res. 6, 463–471.CrossRefPubMedGoogle Scholar
  11. Guillen A. K., Hirai Y., Tanoue T. and Hirai H. 2004 Transcriptional repression mechanisms of nucleolus organizer regions (NORs) in humans and chimpanzees. Chromosome Res. 12, 225–237.CrossRefPubMedGoogle Scholar
  12. Hallenberg C., Nederby-Nielson J., Frederiksen S. 1994 Characterization of 5S rRNA genes from mouse. Gene 142, 291–295.CrossRefPubMedGoogle Scholar
  13. Inafuku J., Nabeyama M., Kikuma Y., Saitoh J., Kubota S. and Kohno S. 2000 Chromosomal location and nucleotide sequences of 5S ribosomal DNA of two cyprinid species (Osteichthyes, Pisces). Chromosome Res. 8, 193–199.CrossRefPubMedGoogle Scholar
  14. Jordan G. 1987 At the heart of the nucleolus. Nature 329, 489–499.CrossRefPubMedGoogle Scholar
  15. Khuda-Bukhsh A. R. and Nayak K. 1982 Karyomorpological studies in two species of hill stream fishes from Kashmir, India: occurrence of a high number of chromosomes. Chromosome Inf. Sci. 33, 12–14.Google Scholar
  16. Kushwaha B., Srivastava S. K., Nagpure N. S., Ogale S. N. and Ponniah A. G. 2001 Cytogenetic studies in two species of mahseer, Tor khudree and Tor mussullah (Cyprinidae, Pisces) from India. Chromosome Sci. 5, 47–50.Google Scholar
  17. Lakra W. S. 1996 Cytogenetic studies on endangered fish species: karyotypes of three species of mahseers, Tor putitora, T. tor and T. khudree (Cyprinidae Pisces). Cytobios 85, 205–218.Google Scholar
  18. Long E. O. and David I. D. 1980 Repeated genes in eukaryotes. Annu. Rev. Biochem. 49, 727–764.CrossRefPubMedGoogle Scholar
  19. Martinez J. L., Moran P., Garcia-Vazquez S. and Pendas A. M. 1996 Chromosomal localization of major and 5S rRNA genes in the European eel (Anguilla anguilla). Cytogenet. Cell Genet. 73, 149–152.CrossRefPubMedGoogle Scholar
  20. Martins C. and Galetti P. M. 1999 Chromosomal localization of 5S rRNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res. 7, 363–367.CrossRefPubMedGoogle Scholar
  21. Martins C. and Galetti P. M. 2000 Conservative distribution of 5s rDNA loci in Schizodon (Pisa, Anastomidae) chromosomes. Chromosome Res. 85, 353–355.CrossRefGoogle Scholar
  22. Martins C. and Galetti P. M. 2001 Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers. Genome 44, 903–910.CrossRefPubMedGoogle Scholar
  23. Martins C. and Wasko A. P. 2004 Organization and evolution of 5S ribosomal DNA in the fish genome. In Focus on genome research (ed. C. R. Williams), pp. 289–319. Nova Science Publishers, New York, USA.Google Scholar
  24. Miklos G. L. G. 1985 Localized highly repetitive DNA sequences in vertebrates and invertebrates genomes. In Molecular evolutionary genetics (ed. R. J. MacIntyre), pp. 241–321. Plenum Press, New York, USA.Google Scholar
  25. Mohindra V., Khulbe R. L., Ponniah A. G. and Lal K. K. 2004 Microsatellite loci to assess genetic variation in Tor putitora. J. Appl. Ichthyol. 20, 466–469.CrossRefGoogle Scholar
  26. Moran P., Martinez J. L., Garcia-Vazquez S. and Pendas A. M. 1996 Sex-linkage of 5S r DNA in rainbow trout (Oncorhynchus mykiss). Cytogenet. Cell Genet. 75, 145–150.CrossRefPubMedGoogle Scholar
  27. Pasolini P., Costagliola D., Rocco L. and Tinti F. 2006 Molecular organization of 5s rDNA in Rajidae (Chondrichthyes): structural features and evolution of piscine 5S rRNA genes and non-transcribed intergenic spacers. J. Mol. Evol. 62, 564–574.CrossRefPubMedGoogle Scholar
  28. Pendas A. M., Moran P. and Garcia-Vazquez E. 1993 Multi chromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chromosome Res. 1, 63–67.CrossRefPubMedGoogle Scholar
  29. Pinhal D., Araki C., Gadig O. B. F. and Martins C. 2009 molecular organization of 5S rDNA in sharks of the genus Rhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes. Genet. Res. 91, 61–72.CrossRefPubMedGoogle Scholar
  30. Ren X., Eisenhour L., Hong C., Lee Y. and McKee B. D. 1997 Roles of rDNA spacer and transcription unit-sequences in X-Y meiotic chromosome pairing in Drosophila melanogaster males. Chromosoma 106, 29–36.CrossRefPubMedGoogle Scholar
  31. Sajdak S. L., Reed K. M. and Phillips R. B. 1998 Intra individual and interspecies variation in the 5S r DNA of coregonid fish. J. Mol. Evol. 46, 680–688.CrossRefPubMedGoogle Scholar
  32. Sambrook J. and Russell I. 2001 Molecular cloning: a laboratory manual, 3rd edition. Cold Spring Harbor Laboratory Press, Plainsveiw, New York, USA.Google Scholar
  33. Santi-Rampazzo A. P., Nishiyama P. B., Ferreira P. E. B. and Martins-Santos I. C. 2008 Intrapopulational polymorphism of nucleolus organizer regions in Serrapinnus notomelas (Characidae, Cheirodontinae) from the Parana River. J. Fish Biol. 72, 1236–1243.CrossRefGoogle Scholar
  34. Tigano C., Rocco L., Ferrito V., Costagliola D., Pappalardo A. M. and Stinso V. 2004 Chromosomal mapping and molecular characterization of ribosomal RNA genes in Lebias fasciata (Teleostei, Cyprinodontidae). Genetica 121, 95–100.CrossRefPubMedGoogle Scholar
  35. Wasko A. P., Martins C., Wright J. M. and Galetti P. M. 2001 Molecular organization of 5S rDNA in fishes of genus Brycon. Genome 44, 893–902.CrossRefPubMedGoogle Scholar
  36. Weiler K. S. and Wakimoto B. T. 1995 Heterochromatin and gene expression in Drosophila. Annu. Rev. Genet. 29, 577–605.CrossRefPubMedGoogle Scholar
  37. Winterfeld G. and Roser M. 2007 Deposition of ribosomal DNAs in the chromosome of perennial oats (Poaceae: Aveneae). Bot. J. Linn. Soc. 155, 193–210.CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2009

Authors and Affiliations

  • Mamta Singh
    • 1
  • Ravindra Kumar
    • 1
  • N. S. Nagpure
    • 1
  • Basdeo Kushwaha
    • 1
  • Indra Mani
    • 1
  • U. K. Chauhan
    • 2
  • W. S. Lakra
    • 1
  1. 1.National Bureau of Fish Genetic ResourcesLucknowIndia
  2. 2.Faculty of Life Science, School of Environmental BiologyAwadhesh Pratap Singh UniversityRewaIndia

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