Journal of Genetics

, Volume 88, Issue 2, pp 165–175 | Cite as

RETRACTED ARTICLE: Dwarf mutations in grass pea (Lathyrus sativus L.): origin, morphology, inheritance and linkage studies

Research Article

Abstract

Induction of mutation has been used to create additional genetic variability in grass pea (Lathyrus sativus L.). During the ongoing investigations on different induced-morphological mutants, the author detected three types of dwarf mutants in grass pea. One mutant, designated as dwf1 type was earlier identified in colchicine-induced C2 generation of grass pea variety BioR-231 while the other two, designated as dwf2 and dwf3 were isolated in 250 Gy and 300 Gy gamma ray irradiated M2 progeny of variety ‘BioR-231’ and ‘Hooghly Local’, respectively. As compared to their parental varieties (controls), all the three mutants manifested stunted, erect and determinate stem, early maturity and tolerance to pod shattering habit. The mutants differed from each other, as well as with controls, in number of primary branches, nature of stipules and internodes, length of peduncle, leaflet and seed coat colour, seed yield and seed neurotoxin content. The three dwarf mutants were monogenically recessive and bred true in successive generations. F2 segregation pattern obtained from the crosses involving the three mutants indicated that dwarf mutation in grass pea was controlled by two independent non-allelic genes, assigned as df1 (for dwf1 type), df2 (for dwf2 type) and df3 (for dwf3 type), with the df1 locus being multiple allelic. Primary trisomic analyses revealed the presence of df1/df2 locus on the extra chromosome of trisomic type I, whereas df3 was located on the extra chromosome of type III. Linkage studies involving five other phenotypic markers suggested linked association of df1/df2 locus with lfc (leaflet colour) and wgn (winged internode) and df3 locus with cbl (seed coat colour). Both the loci; however, assorted independently with flower colour and stipule character. The dwarf types can be utilized as valuable tools for further cytogenetic research and breeding of grass pea.

Keywords

dwarf mutations inheritance linkage trisomic analysis grass pea (Lathyrus sativus L.) 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Biswas A. K. 2007 Induced mutation in grass pea. In Breeding of neglected and under-utilized crops, spices and herbs (ed. S. Ochatt and S. M. Jain), pp. 29–39. Science Publishers, Enfield.Google Scholar
  2. Biswas S. C. 1998 Cytogenetic evaluation of induced variations in Lathyrus sativus L. through mutation and polyploidy and varietal diversities. Ph.D. thesis, University of Kalyani, Kalyani.Google Scholar
  3. Chowdhury M. A. and Slinkard A. E. 2000 Inheritance and linkage of isozymes in grass pea. Lathyrus Lathyrism Newslett. 1, 1–4.Google Scholar
  4. Das P. K. and Kundagrami S. 1999 Inheritance of flower colour in grass pea. Ind. J. Genet. 59, 117–118.Google Scholar
  5. Dirks V. A., Ross J. G. and Harpstead D. D. 1956 Colchicine induced true-breeding chemical sectors in flax. J. Hered. 47, 229–233.CrossRefGoogle Scholar
  6. Downes R. W. and Marshall D. R. 1983 Colchicine-induced variants in sunflower. Euphytica 32, 757–766.CrossRefGoogle Scholar
  7. FAO 2002 Lathyrus sativus L. In Crop plant, pp. 1–3. Rome, Italy.Google Scholar
  8. Foster A. E., Ross J. G. and Franzke C. J. 1961 Estimates of the number of mutated genes in a colchicine induced mutant of sorghum. Crop Sci. 1, 272–276.CrossRefGoogle Scholar
  9. Gaur P. M., Gour V. K. and Srinivasan S. 2008 An induced brachytic mutant of chickpea and its possible use in ideotype breeding. Euphytica 159, 35–41.CrossRefGoogle Scholar
  10. Gutierrez J. F., Vaquero F. and Vences F. J. 2001 Genetic mapping of isozyme loci in Lathyrus sativus L. Lathyrus Lathyrism. Newslett. 2, 74–78.Google Scholar
  11. Honeycutt R. J., Newhouse K. E. and Palmer R. G. 1989 Inheritance and linkage studies of a variegated leaf mutant in soybean. J. Hered. 81, 123–126.Google Scholar
  12. Jain H. K. 1975 Breeding for yield and other attributes in grain legumes. Ind. J. Genet. 35, 169–187.Google Scholar
  13. Khanna-Chopra R. and Sinha S. K. 1990 What limits the yield of pulses? Plant processes or plant type. In Proceedings of the international congress in plant physiology, vol. 1 (ed. S. K. Sinha., P. V. Sane., S. C. Bhargava and P. K. Agarwal), pp. 268–278. Indian Agricultural research Institute, New Delhi.Google Scholar
  14. Khush G. S., Singh R. J., Sur S. C. and Librojo A. L. 1984 Primary trisomics of rice: origin, morphology, cytology and use in linkage mapping. Genetics 107, 141–163.PubMedPubMedCentralGoogle Scholar
  15. Kolb F. L. and Marshall H. G. 1984 Peduncle elongation in dwarf and normal height oats. Crop Sci. 24, 699–703.CrossRefGoogle Scholar
  16. Kosambi D. D. 1944 The estimation of map units from recombination values. Ann. Eugen. (London) 12, 172–175.CrossRefGoogle Scholar
  17. Lather V. S. 2000 Promising chickpea ideotype for higher plant density. Int. Chickpea Pigeonpea Newslett. 7, 26–27.Google Scholar
  18. Lesley J. W. 1926 The genetics of Lycopersicum esculentum Mill. 1. The trisomic inheritance of ‘Dwarf’. Genetics 11, 352–354.PubMedPubMedCentralGoogle Scholar
  19. McCutchan J. S. 2003 Review: a brief history of grass pea and its use in crop improvement. Lathyrus Lathyrism. Newslett. 3, 21–25.Google Scholar
  20. Mehra R. B., Raju D. B. and Himabindu K. 1995 Evaluation and utilization of Lathyrus sativus collection in India. In Lathyrus genetic resources in India (R. K. Arora, P. N. Mathur, K. W. Riley and Y. Adham) pp. 37–43. Indira Gandhi Agricultural University, Raipur.Google Scholar
  21. Qin R., Qiu Y., Cheng Z., Shan X., Guo X., Zhai H. and Wan J. 2008 Genetic analysis of a novel dominant rice dwarf mutant 986083D. Euphytica 160, 379–387.CrossRefGoogle Scholar
  22. Rai K. N. and Hanna W. W. 1990 Morphological characteristics of tall and dwarf pearl millet isolines. Crop Sci. 30, 23–25.CrossRefGoogle Scholar
  23. Rao S. L. N. 1978 A sensitive and specific colorimetric method for the determination of α, β-diamino propionic acid and the Lathyrus sativus neurotoxin. Anal. Biochem. 86, 386–395.CrossRefGoogle Scholar
  24. Rybinski W. 2003 Mutagenesis as a tool for improvement of traits in grass pea (Lathyrus sativus L.). Lathyrism Lathyrism. Newslett. 3, 30–34.Google Scholar
  25. Sethi G. S. 1974 Long-peduncled mutant: a new mutant type induced in barley. Euphytica 23, 237–239.CrossRefGoogle Scholar
  26. Skiba B., Ford R. and Pang E. C. 2004 Construction of a linkage map based on a Lathyrus sativus backcross population and preliminary investigation of QTLs associated with resistance to ascochyta blight. Theor. Appl. Genet. 109, 1726–1735.CrossRefGoogle Scholar
  27. Smartt J. 1984 Evolution of grain legumes. I. Mediterranean pulses. Exp. Agric. 20, 275–296.CrossRefGoogle Scholar
  28. Talukdar D. and Biswas A. K. 2005a Induced seed coat colour mutations and their inheritance in grass pea (Lathyrus sativus L.). Ind. J. Genet. 65, 135–136.Google Scholar
  29. Talukdar D. and Biswas A. K. 2005b Multiple alleles controlling stipule characters in grass pea (Lathyrus sativus L.). In Proceedings of the 4th international food legume research conference (ed. M. C. Kharkwal), pp. 200, Indian Society of Genetics and Plant Breeding (ISGPB), New Delhi.Google Scholar
  30. Talukdar D. and Biswas A. K. 2006 An induced internode mutant in grass pea. In Perspectives in cytology and genetics, vol. 12 (ed. R. K. Das., S. Chatterjee and G. C. Sadhukhan), pp. 267–271. AICCG publication, Kalyani.Google Scholar
  31. Talukdar D. and Biswas A. K. 2007a A long pedicelled mutant and its inheritance in grass pea. Ind. J. Genet. 67, 85–86.Google Scholar
  32. Talukdar D. and Biswas A. K. 2007b Seven different primary trisomics in grass pea (Lathyrus sativus L.). I Cytogenetic characterization. Cytologia 72, 385–396.CrossRefGoogle Scholar
  33. Talukdar D. and Biswas A. K. 2007c Inheritance of flower and stipule characters in different induced mutant lines of grass pea (Lathyrus sativus L.). Ind. J. Genet. 67, 396–400.Google Scholar
  34. Talukdar D., Biswas S. C. and Biswas A. K. 2001a Induced mutation in grass pea (Lathyrus sativus L.). In Perspectives in cytology and genetics, vol. 10 (ed. G. K. Manna and S. C. Roy), pp. 481–484. AICCG publication, Kalyani.Google Scholar
  35. Talukdar D., Biswas S. C. and Biswas A. K. 2001b An induced dwarf mutant of grass pea. Ind. J. Genet. 61, 383–384.Google Scholar
  36. Talukdar D., Biswas S. C. and Biswas A. K. 2002 An induced flower colour mutant in grass pea (Lathyrus sativus L). Ind. J. Genet. 62, 162.Google Scholar
  37. Tiwari K. R. and Campbell C. G. 1996 Inheritance of neurotoxin (ODAP) content, flower and seed coat colour in grass pea (Lathyrus sativus L.). Euphytica 91, 195–203.Google Scholar
  38. Tsuchiya T. and Haines R. 1975 Trisomic analysis of nine mutant genes in barley. Barley Genet. Newslett. 5, 67–69.Google Scholar
  39. Waghmare V. N. and Mehra R. B. 2000 Induced genetic variability for quantitative characters in grass pea (Lathyrus sativus L.). Ind. J. Genet. 60, 81–87.Google Scholar
  40. Weigle J. L. and Butler J. K. 1983 Induced dwarf mutant in Impatiens platypetala. J. Hered. 74, 200.CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2009

Authors and Affiliations

  1. 1.Department of BotanyUniversity of KalyaniKalyaniIndia

Personalised recommendations