Molecular assessment of genetic diversity in mung bean germplasm
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RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for Jaccard’s similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level. Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package. The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appears to be related to their performance in field conditions for 10 morphological traits that were scored. This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.
Keywordsgenetic diversity local land races mung bean field performance RAPD
- Arunachalam V. 1981 Genetic distance in plant breeding. Indian J. Genet. 14, 226–236.Google Scholar
- Chattopadhyay K., Ali M. N., Sarkar H. K., Mandal N. and Bhattacharyya S. 2005 Diversity analysis by RAPD and ISSR markers among the selected mungbean [Vigna radiata (L.) Wilczek] genotypes. Indian J. Genet. Pl. Breed. 65, 173–175.Google Scholar
- Manivannan N., Murugan E., Viswanathan P. L. and Dhanakodi C. V. 1998 Genetic divergence in greengram. Legume Res. 21, 131–133.Google Scholar
- Mignouna H. D., Ng N. Q., Ikea J. and Thotapilly G. 1998 Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J. Genet. Breed. 53, 151–159.Google Scholar
- Page R. D. M. 2001 TreeView (Windows 32) version 1.6.5. Distributed by author. http://taxonomy.zoology.gla.ac.uk/rod/rod.html.
- Pavlicek A., Hrda S. and Flegr J. 1999 Free Tree — Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrapping / jackknife analysis of the tree robustness. Application in the RAPD analysis of the genus Frenkelia. Folia Biol. (Praha.) 45, 97–99.Google Scholar
- Ramanujam S. 1981 Biometrical basis for yield improvement in mungbean. In: Procedings 1st International Mung bean Symposium, (ed. R. Cowell) pp. 210 AVRDC, Shanhua.Google Scholar
- Saini A., Reddy K. S. and Jawali N. 2004 Evaluation of long primers for AP-PCR analysis of mungbean [Vigna radiata (L.) Wilczek]: Genetic relationships and fingerprinting of some genotypes. Indian J. Biotechnol. 3, 511–518.Google Scholar
- Skroch P. W., dos Santos J. B. and Nienhuis J. 1992 Genetic relationships among Phaseolus vulgaris genotypes based on RAPD marker data. Annu. Rep. Bean Improv. Coop. 35, 23–24.Google Scholar
- Yee E., Kidwell K. K., Sills G. R. and Lumpkin, T. A. 1999 Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sci. 39, 268–275.Google Scholar