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A study on fluoride bioremediation via a novel bacterium Bacillus megaterium (JF273850) isolated from agricultural soil

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Abstract

Micro-organisms often exhibit tolerance to a wide range of pollutants for their existence in different adverse environments. Fluoride is one of the potential geogenic contaminants setting up a great ecological concern in the world as well as in India. It is a protoplasmic toxin and minimal amount of it can initiate several biochemical changes in micro-organisms. The objectives of the present investigation were to isolate fluoride-resistant bacteria from the contaminated agricultural soil matrix of fluoride-prone area and to evaluate the fluoride contamination status of soil and groundwater. The fluoride level was found to be in the range of 1.98–2.32 mg/kg and 0.17–5.33 mg/L in the soil and groundwater, respectively. A new fluoride-resistant bacterial strain (NM27) was isolated from the agricultural soil. The 16S rRNA gene sequencing, phylogenetic analysis, and phenotypic characterisation revealed that the bacterial isolate is very similar to Bacillus megaterium (JF273850). The G+C and A+T content of the 16S rDNA was found to be 53.34 and 46.66 mol%, respectively. The isolated strain showed positive tests for catalase, lipase, urease, protease, oxidase, and H2S production along with sensitivity towards the recommended doses of various antibiotics. This bacterial isolate can survive both in high level of fluoride (1500 mg/L) and sodium chloride (35% w/v) solution and in a medium having wide pH range (4–12). The 16S rRNA gene sequence of the bacterial isolate, NM27 has been deposited in the NCBI Gene bank and was assigned the accession number (JF273850). Fluoride bioaccumulation study suggested that this particular strain (NM27) can reduce the fluoride concentration in the culture medium up to 70%. Therefore, it can be concluded that bacterial strain Bacillus megaterium could be used for bioremediation of fluoride.

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Acknowledgements

The authors acknowledge the funding agency, WBDST Memo No: 126 [Sanc.]/ST/P/S&T/15G-10/2015 and DST-FIST, Govt. of India [No. SR/FST/ESI-141/2015(2) Dt. 30th Sept. 2019] for providing necessary funds for conducting the present research. The authors are also thankful to all supporting staff members who directly or indirectly helped in shaping the present work and to the faculty members of the Department of Environmental Science, Burdwan University, India.

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Authors and Affiliations

  1. Department of Chemistry, Karimpur Pannadevi College, Karimpur, 741 152, Nadia, India

    Kartick Chandra Pal

  2. Parasitology and Microbiology Laboratory, Department of Zoology, The University of Burdwan, Burdwan, 713 104, India

    Priyanka Mukhopadhyay & Soumendranath Chatterjee

  3. Environmental Chemistry Laboratory, Department of Environmental Science, The University of Burdwan, Burdwan, 713 104, India

    Naba Kumar Mondal

Authors
  1. Kartick Chandra Pal
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  2. Priyanka Mukhopadhyay
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  3. Soumendranath Chatterjee
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  4. Naba Kumar Mondal
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Contributions

Naba Kumar Mondal and Soumendranath Chatterjee planned and supervised the experiments on defluorination by using identified bacterial sp. and verified the manuscript. Kartick Chandra Pal and Priyanka Mukhopadhyay executed the experiment and prepared the manuscript.

Corresponding author

Correspondence to Naba Kumar Mondal.

Additional information

Communicated by Santanu Banerjee

Supplementary material pertaining to this article is available on the Journal of Earth System Science website (http://www.ias.ac.in/Journals/Journal_of_Earth_System_Science).

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Pal, K.C., Mukhopadhyay, P., Chatterjee, S. et al. A study on fluoride bioremediation via a novel bacterium Bacillus megaterium (JF273850) isolated from agricultural soil. J Earth Syst Sci 131, 183 (2022). https://doi.org/10.1007/s12040-022-01931-z

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  • DOI: https://doi.org/10.1007/s12040-022-01931-z

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