Skip to main content
SpringerLink
Log in

Microdetermination of human serum albumin by differential pulse voltammetry at a l-cysteine modified silver electrode

  • Published:
Journal of Chemical Sciences Aims and scope Submit manuscript

Abstract

A simple and highly sensitive electrochemical method for the determination of human serum albumin (HSA) using differential pulse voltammetry (DPV), based on a silver electrode modified with a self-assembled monolayer of l-cysteine, was developed. l-cysteine can be modified onto a silver electrode by covalent bonding through the sulfur to give stable and long-lived chemical electrodes. This electrode showed good sensitivity, selectivity, reproducibility and time stability in the determination of trace amounts of HSA by DPV technique. The detection limit can be as low as 4 × 10−17 mol/L. The optimum conditions for the determination were carefully investigated. This method had been applied to the determination of HSA in human serum samples. The results were in agreement with those given in standard method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Joo I S, Lee S H, Suh J K and Kim C J 2002 J. Anal. Sci. 17 117

    Google Scholar 

  2. Hara T, Yokogi J, Okamura S, Kato S and Nakajima R 1993 J. Chromatog. A652 361

    Article  Google Scholar 

  3. Li Z, Li K and Tong S 1998 Microchem. J. 60 217

    Article  CAS  Google Scholar 

  4. Wei Y J, Li K A and Tong S Y 1996 J. Talanta. 43 1

    Article  CAS  Google Scholar 

  5. Zhang L, Li N, Zhao F L and Li K A 2004 J. Anal. Sci. 20 445

    Article  CAS  Google Scholar 

  6. Lee S H, Suh J K and Li M 2003 J. Bull. Korean Chem. Soc. 24 45

    Article  CAS  Google Scholar 

  7. Li D H, Yang H H, Zhen H, Fang Y, Zhu Q Z and Xu J G 1999 J. Anal. Chim. Acta 401 185

    Article  CAS  Google Scholar 

  8. Ma C, Li C and Tong S 1997 J. Anal. Chim. Acta 338 255

    Article  CAS  Google Scholar 

  9. Huang C, Li Y, Mao J and Tan D 1998 J. Analyst. 123 1401

    Article  Google Scholar 

  10. Yao G, Li K and Tong S 1999 J. Anal. Chim. Acta 398 319

    Article  CAS  Google Scholar 

  11. Wang J, Vila V and Tapia T 1988 J. Bioeletrochem. Bioenerg. 19 39

    Article  CAS  Google Scholar 

  12. Estela J M, Tomas C, Cladera A and Cerda V 1995 J. Crit. Rev. Anal. Chem. 259 91

    Article  Google Scholar 

  13. Honeychurch M J and Ridd M J 1996 J. Electroanalysis 8 654

    Article  CAS  Google Scholar 

  14. Honeychurch M J and Ridd M J 1996 J. Electroanalysis. 8 49

    Article  CAS  Google Scholar 

  15. Tomschik M, Havran L, Fojta M and Palecek E 1998 J. Electroanalysis 10 403

    Article  CAS  Google Scholar 

  16. Anderson J L, Bowden E F and Pickup P G 1996 J. Anal. Chem. 68 413R

    Google Scholar 

  17. Sun W, Han J Y, Yong R and Jiao K 2006 J. Braz. Chem. Soc. 17 510

    Article  CAS  Google Scholar 

  18. Sun W, Han J Y, Jiao K and Lu L D 2006 Chin. J. Anal. Sci. 22 145

    CAS  Google Scholar 

  19. Sun W, Han J Y and Jiao K 2005 Chin. J. Anal. Lab. 24 59

    CAS  Google Scholar 

  20. Lin L, Qiu P H, Yang L Z and Cao X N 2006 Chin. J. Anal. Chem. 34 31

    CAS  Google Scholar 

  21. Lu Z, Huang Q and Rusling J F 1997 J. Electroanal. Chem. 423 59

    Article  CAS  Google Scholar 

  22. Ma X Y, Chao Z, Li X, Xu H Y and Zhang G R 2006 Chin. J. South Med. Univ. 26 648

    CAS  Google Scholar 

  23. Jia L, Zhen Y, Zhang X H and Wang S F 2004 Chin. J. Hubei Univ. (Nat. Sci. Edit.) 26 48

    CAS  Google Scholar 

  24. Li G, Fang H, Long Y, Chen H and Zhu D 1996 J. Anal. Lett. 29 1273

    CAS  Google Scholar 

  25. Gu K, Zhu J J and Chen H Y 1999 Chin. J. Anal. Chem. 27 1172

    CAS  Google Scholar 

  26. Long E C and Barton J K 1990 Acc. Chem. Res. 23 271

    Article  CAS  Google Scholar 

  27. Stankovich M T and Bard A J 1978 J. Electroanal. Chem. 86 189

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Huaibei Coal Normal College, Huaibei, 235000, China

    Liyuan Lu, Yanqin Zi & Hongling Wang

Authors
  1. Liyuan Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanqin Zi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongling Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yanqin Zi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lu, L., Zi, Y. & Wang, H. Microdetermination of human serum albumin by differential pulse voltammetry at a l-cysteine modified silver electrode. J Chem Sci 120, 419–424 (2008). https://doi.org/10.1007/s12039-008-0066-4

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12039-008-0066-4

Keywords

Search

Navigation