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Polyamines conjugated to deoxyribonucleic acid-protein in cell nucleus from filling grain embryos were involved in tolerance of wheat to drought

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Abstract

Deoxyribonucleic acid-protein (DNAP) of the cell nucleus was purified from developing wheat (Triticum aestivum L.) embryo cells under drought stress, with two cultivars differing in drought tolerance as experimental materials – Longmai No. 079 (drought-tolerant) and Wanmai No. 52 (drought-sensitive). Levels of polyamines (PAs) non-covalently conjugated to the DNA and covalently conjugated to the proteins of DNAP were detected. After soil drought treatment for 10 days, in drought-tolerant Longmai No. 079, the increases in the levels of spermine and spermidine non-covalently conjugated to DNA of DNAP were more statistically significant (P<0.05) than in drought-sensitive Wanmai No. 52. Treatment of Wanmai No. 52 with exogenous Spm could not only enhance the tolerance of the cultivar to drought stress, as judged by flag leaf water content, plasma membrane permeability and grain growth, but also elevate the levels of spermine and spermidine non-covalently conjugated to the DNA of the cultivar. On the contrary, treatment of Longmai No. 079 with methylglyoxyl-bis guanylhydrazone, an inhibitor of S-adenosylmethionine decarboxylase, could significantly (P<0.05) aggravate the drought stress to this cultivar, accompanied by a marked decreases in the levels of spermine and spermidine non-covalently conjugated to the DNA of the cultivar. On the other hand, the content of putrescine covalently conjugated to the proteins of DNAP rose more markedly (P<0.05) in Longmai No. 079 than in Wanmai No. 52. The transglutaminase inhibitor, o-phenanthrolin, could markedly reduce the drought-induced increase in the level of putrescine covalently conjugated to the proteins of DNAP and aggravate drought stress to the two cultivars. Collectively, it could be inferred that spermine and spermidine non-covalently conjugated to the DNA and putrescine covalently conjugated to the proteins of DNAP in the developing grain embryo cell nucleus might enhance the tolerance of wheat plants to soil drought.

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References

  • Antony T, Thomas T and Shirahata A 1999 Selectivity of polyamines on the stability of RNA-DNA hybrids containing phosphodiestor and phosphorothioate oligodeoxyribonucleotides. Biochemistry 28 10775–10784

    Article  Google Scholar 

  • Beshamgan ES, Sharifi M and Zarinkamar F 2019 Crosstalk among polyamines, phytohormones, hydrogen peroxide, and phenylethanoid glycosides responses in Scrophularia striata to Cd stress. Plant Physiol. Biochem. 143 129–141

    Article  CAS  Google Scholar 

  • Bradford MM 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binging. Anal. Biochem. 72 248–254

    Article  CAS  Google Scholar 

  • Del Duca S, Serafini-Fracassini D and Cai G 2014 Senescence and programmed cell death in plants: Polyamine action mediated by transglutaminase. Front. Plant Sci. 5 120

    Google Scholar 

  • Du HY, Chen GS, Yu JM, et al. 2019 Involvement of putrescine in osmotic stress-induced ABA signaling in leaves of wheat seedlings. J. Biosci. 44 136

    Article  Google Scholar 

  • Du HY, Liu GT, Liu DX, et al. 2020 Polyamines conjugated to embryo tonoplast enhance the tolerance of maize to drought stress by maintaining the vacuole conformation. Russ. J. Plant Physiol. 67 724–732

    Article  CAS  Google Scholar 

  • Du HY, Liu GT, Hua CM, et al. 2021 Exogenous melatonin alleviated chilling injury in harvested plum fruit via affecting the levels of polyamines conjugated to plasma membrane. Postharvest Biol. Tec. 179 111585

    Article  CAS  Google Scholar 

  • Du HY, Liu DX, Liu GT, et al. 2022 Conjugated polyamines are involved in conformation stability of plasma membrane from maturing maize grain embryos under drought stress. Environ. Exp. Bot. 194 104726

    Article  CAS  Google Scholar 

  • Dutra NT, Silveira V, Azevedo IG, et al. 2013 Polyamines affect the cellular growth and structure of pro-embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines. Physiol. Plant. 148 121–132

    Article  CAS  Google Scholar 

  • Ebmeyer H, Fiedler-Wiechers K and Hoffmann CM 2021 Drought tolerance of sugar beet–evaluation of genotypic differences in yield potential and yield stability under varying environmental conditions. Eur. J. Agron. 125 126262

    Article  Google Scholar 

  • Farooq M, Wahid A and Lee DJ 2009 Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiol. Plant. 31 937–945

    Article  CAS  Google Scholar 

  • Gondor OK, Tajti J, Hamow KÁ, et al. 2021 Polyamine metabolism under different light regimes in wheat. Int. J. Mol. Sci. 22 11717

    Article  CAS  Google Scholar 

  • Ha HC, Sirisoma NS and Kuppusamy P 1998 The natural polyamine spermine functions directly as a free radical scavenger. Proc. Natl. Acad. Sci. USA 95 11140–11145

    Article  CAS  Google Scholar 

  • Hashem AM, Moore S, Chen S, et al. 2021 Putrescine depletion affects arabidopsis root meristem size by modulating auxin and cytokinin signaling and ROS accumulation. Int. J. Mol. Sci. 22 4094

    Article  CAS  Google Scholar 

  • Icekson I and Apelbaum A 1987 Evidence for transglutaminase activity in plant tissue. Plant Physiol. 84 972–974

    Article  CAS  Google Scholar 

  • Jing JG, Guo SY, Li YF and Li WH 2020 The alleviating effect of exogenous polyamines on heat stress susceptibility of different heat resistant wheat (Triticum aestivum L.) varieties. Sci. Rep. 10 7467

  • Kaur-Sawhney R and Shin M 1982 Relation of polyamines synthesized titer to aging and senescence in oat leaves. Plant Physiol. 69 405–410

    Article  CAS  Google Scholar 

  • Lechowska K, Wojtyla Ł, Quinet M, et al. 2022 Endogenous polyamines and ethylene biosynthesis in relation to germination of osmoprimed brassica napus seeds under salt stress. Int. J. Mol. Sci. 23 349

    Article  CAS  Google Scholar 

  • Liu DX, Liu HL, Du HY, Liu HP and Kurtenbach R 2021 Relationship between polyamines conjugated to mitochondrion membrane and mitochondrion conformation from developing wheat embryos under drought stress. J. Biosci. 46 31

    Article  CAS  Google Scholar 

  • Liu HP, Liu YL, Yu BJ, Liu ZP and Zhang WH 2004 Increased polyamines conjugated to tonoplast vesicles correlate with maintenance of the H+-ATPase and H+-PPase activities and enhanced osmotic stress tolerance in wheat. J. Plant Growth Regul. 23 156–165

    Article  CAS  Google Scholar 

  • Liu HP, Ji XE, Yu BJ and Liu YL 2005 Relationship between osmotic stress and polyamines conjugated to the deoxyribonucleic acid-protein in wheat seedling roots. Sci. in China (Ser. C-Life sci.) 49. CNKI:SUN:JCXG.0.2006-01-001

  • Lutts S, Hausman J, Quinet M and Lefèvre I 2013 Polyamines and their roles in the alleviation of ion toxicities in plants; in: Ecophysiology and Responses of Plants under Salt Stress (eds) P Ahmad, M Azooz and M Prasad (New York, NY, USA: Springer) pp 315–353

  • Pál M, Szalai G, Gondor OK and Janda T 2021 Unfinished story of polyamines: role of conjugation, transport and light-related gegulation in the polyamine metabolism in plants. Plant Sci. 308 110923

    Article  Google Scholar 

  • Pottosin I, Olivas-Aguirre M, Dobrovinskaya O, Zepeda-Jazo I and Shabala S 2021 Modulation of ion transport across plant membranes by polyamines: Understanding specific modes of action under stress. Front. Plant Sci. 11 616077

    Article  Google Scholar 

  • Rakić T, Pešić M, Kostić N, et al. 2021 Rhizobacteria associated with Miscanthus x Giganteus improve metal accumulation and plant growth in the flotation tailings. Plant Soil 462 349–363

    Article  Google Scholar 

  • Sobieszczuk-Nowicka E, Paluch-Lubawa E, Mattoo AK, et al. 2019 Polyamines—A new metabolic switch: Crosstalk with networks involving senescence, crop improvement, and mammalian cancer therapy. Front. Plant Sci. 10 859

    Article  Google Scholar 

  • Szalai G, Janda K, Darkó É, et al. 2017 Comparative analysis of polyamine metabolism in wheat and maize plants. Plant Physiol. Biochem. 112 239–250

    Article  CAS  Google Scholar 

  • Tiburcio AF, Campos JL and Figueras X 1993 Recent advances in the understanding of polyamines functions during plant development. Plant Growth Regul. 12 331–340

    Article  CAS  Google Scholar 

  • Yang JC, Zhang JH, Liu K, Wang ZQ and Liu LJ 2007 Involvement of polyamines in the drought resistance of rice. J. Exp. Bot. 58 1545–1555

    Article  CAS  Google Scholar 

  • Zhong M, Song R, Wang Y, et al. 2020 TGase regulates salt stress tolerance through enhancing bound polyamines-mediated antioxidant enzymes activity in tomato. Environ. Exp. Bot. 179 104191

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no.: 31271627) and Natural Science Foundation of Henan province (Grant no.: 222300420394).

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Authors and Affiliations

  1. College of Life Science and Agronomy/Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou Normal University, Zhoukou, 466001, Henan, China

    H Y Du, D X Liu, H P Liu & R Kurtenbach

  2. School of Life Sciences, East China Normal University, Shanghai, 200062, China

    H L Liu

  3. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225000, Jiangsu, China

    D X Liu

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  1. H Y Du
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  2. H L Liu
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  3. D X Liu
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  4. H P Liu
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  5. R Kurtenbach
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Correspondence to H P Liu.

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Corresponding editor: Manchikatla Venkat Rajam

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Du, H.Y., Liu, H.L., Liu, D.X. et al. Polyamines conjugated to deoxyribonucleic acid-protein in cell nucleus from filling grain embryos were involved in tolerance of wheat to drought. J Biosci 47, 85 (2022). https://doi.org/10.1007/s12038-022-00319-x

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  • DOI: https://doi.org/10.1007/s12038-022-00319-x

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