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CircRNA expression profiling and bioinformatics analysis indicate the potential biological role and clinical significance of circRNA in influenza A virus-induced lung injury

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Abstract

Circular RNA (circRNA) plays an important role in the regulation of multiple biological processes. However, circRNA profiling and the potential biological role of circRNA in influenza A virus (IAV)-induced lung injury have not been investigated. In the present study, circRNA expression profiles in lung tissues from mice with and without IAV-induced lung injury were analyzed using high-throughput sequencing, and differentially expressed circRNAs were verified by quantitative PCR. The gene homology of candidate circRNAs was investigated and the expression of plasma circRNAs from patients with IAV-induced acute respiratory distress syndrome (ARDS) was detected. The target microRNAs (miRNAs) of circRNAs were predicted. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. In total, 781 circRNAs were differentially expressed between ARDS mice and control (467 were up-regulated and 314 were down-regulated). Moreover, the candidate circRNAs (Slco3a1, Nfatc2, Wdr33, and Dmd) expression showed the same trend with the sequencing results. The isoforms of circRNA Slco3a1 and Wdr33 were highly conserved between humans and mice. Plasma circRNA Slco3a1 and Wdr33 presented differential expression in patients with IAV-induced ARDS compared to control. The circRNA-miRNA interaction network and GO and KEGG analyses indicated the potential biological role of circRNAs in the development of IAV-induced lung injury. Taken together, a large number of differentially expressed circRNAs were identified in our study. CircRNA Slco3a1 and Wdr33 had significantly different expression in specimens from mice and humans, and showed a potential biological role in IAV-induced lung injury by bioinformatics analysis.

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Abbreviations

ARDS:

acute respiratory distress syndrome

circRNA:

circular RNA

GO:

gene ontology

IAV:

influenza A virus

KEGG:

Kyoto encyclopedia of genes and genomes

miRNA:

MicroRNA

qPCR:

quantitative PCR

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Acknowledgements

This work was supported by grants from the National Science and Technology Major Project (No. 2017ZX10204401003), the Suzhou Science and Technology Project (Nos. KJXW2018008, SYS2019048, and SS201711), and the Suzhou Medical Key Subject (SZXK201516). We would like to thank Dr. Anushka Aghi, from Panchkula, India, for her help in the development of this manuscript.

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Author notes

  1. Jiajia Wang and Yanbing Zhang have equally contributed.

Authors and Affiliations

  1. Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

    Jiajia Wang, Qingqing Zhu, Junhong Jiang & Jian-an Huang

  2. Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

    Yanbing Zhang

  3. Department of Emergency and Critical Care Medicine, The Fifth People’s Hospital of Suzhou, Suzhou, 215000, China

    Fengfeng Zhu

  4. Suzhou Center for Disease Control and Prevention, Suzhou, 215004, China

    Liling Chen

  5. Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

    Yao Wei, Qiang Guo & Xinjing Yang

Authors
  1. Jiajia Wang
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  2. Yanbing Zhang
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  3. Fengfeng Zhu
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  4. Liling Chen
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  8. Jian-an Huang
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  9. Qiang Guo
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  10. Xinjing Yang
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Corresponding authors

Correspondence to Qiang Guo or Xinjing Yang.

Ethics declarations

This study was approved by the Institutional Review Board of the First Affiliated Hospital of Soochow University, and informed consents were obtained from all participants.

Additional information

Corresponding editor: Sreenivas Chavali

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Wang, J., Zhang, Y., Zhu, F. et al. CircRNA expression profiling and bioinformatics analysis indicate the potential biological role and clinical significance of circRNA in influenza A virus-induced lung injury. J Biosci 46, 38 (2021). https://doi.org/10.1007/s12038-021-00152-8

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  • DOI: https://doi.org/10.1007/s12038-021-00152-8

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