Abstract
Nanoparticles (NPs) synthesized by laser ablation in distilled water were used to study their biological effect on normal and cancer cells. Parameters such as cell morphology, cell proliferation and viability were examined for treated cell lines, and the effect was represented in terms of cells cytotoxicity using standard procedures. The study reveals the higher cytotoxic effect of nanoparticles on cancerous cells of breast, melanoma and colon origin compared to normal fibroblast cells NIH-3T3. Furthermore, DNA fragmentation assay results demonstrated the apoptosis mediated cell death in nanoparticle-treated cancer cells. The distinct role of nanoparticles in normal and cancer cells of different origin showed that nanoparticles were specific to cause cytotoxicity in particular cancer cells type. NPs exhibit cytotoxic effects in cancer cells by inducing apoptosis. These studies provide fundamental evidence for the easy, simple and safe mode of nanoparticles synthesis and their application in cancer cells death.
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Acknowledgements
The authors thank UGC, India, for their support to the University of Mysore through the UPE and CPEPA major projects. NKK is thankful to the Department of Science & Technology, India, for INSPIRE Faculty award.
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Communicated by B J Rao.
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Mahadevaiah, Kurrey, N.K., Gowtham, G.K. et al. Laser-ablation-synthesized nanoparticles and animal cell lines studies. J Biosci 44, 135 (2019). https://doi.org/10.1007/s12038-019-9956-5
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DOI: https://doi.org/10.1007/s12038-019-9956-5