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Involvement of putrescine in osmotic stress-induced ABA signaling in leaves of wheat seedlings

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Abstract

To elucidate one mechanism by which putrescine (Put) functions in plant signaling under osmotic stress, Put and ABA contents, and plasma membrane-NADPH oxidase (PM-NOX) activity were detected in wheat seedling leaves. Under osmotic stress, ABA and Put contents, PM-NOX activity, and PM-NOX-dependent O.−2 production all increased. The inhibitor tungstate (T) of ABA bio-synthesis reduced the increases in ABA and Put contents under osmotic stress. The inhibitor D-arginine (D-Arg) of Put bio-synthesis didn’t reduce osmotic-induced increase of ABA, but it inhibited the increases of PM-NOX activity and O. −2 production, and the inhibitory effects were reversed by exogenous Put. These findings suggested that ABA might regulate Put biosynthesis, and Put might regulate PM-NOX activity. Treatments with three inhibitors imidazole (I), diphenylene iodonium (DPI) and pyridine (P) of PM-NOX reduced significantly not only O. −2 production, but also the stress-induced increase of Put content, which indicated that O. −2 production might regulate Put biosynthesis. Treatments with EGTA (Ca2+ chelator), La3+ and verapamil (V) (Ca2+ channel blockers) reduced significantly the stress-induced increase of Put content, which suggested that Ca2+ might regulate Put biosynthesis. With these findings, it could be concluded that Put was involved in ABA signaling induced by osmotic stress via regulating PM-NOX activity in wheat seedling leaves.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No.: 31271627) and Science and Technology Project of Henan Province (Grant No.: 192102110135).

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Authors and Affiliations

  1. College of Life Science and Agronomy/Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou Normal University, Zhoukou, 466001, Henan, China

    HY Du, JM Yu, YY Bao, HP Liu & R Gupta

  2. School of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang, 453000, Henan, China

    GS Chen

  3. College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    GT Liu

Authors
  1. HY Du
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  2. GS Chen
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  3. JM Yu
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  4. YY Bao
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  5. GT Liu
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  6. HP Liu
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Correspondence to HP Liu.

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Corresponding editor: Manchikatla Venkat Rajam

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Du, H., Chen, G., Yu, J. et al. Involvement of putrescine in osmotic stress-induced ABA signaling in leaves of wheat seedlings. J Biosci 44, 136 (2019). https://doi.org/10.1007/s12038-019-9949-4

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  • DOI: https://doi.org/10.1007/s12038-019-9949-4

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