Abstract
The importance of studying microbial load on fabrics has been recently realized with reports on fabrics being a source of spread of infection in medical and hospitality sectors. However, methodological limitations have restricted the analysis of microbial diversity on fabrics. Hence, the study aimed to develop a robust method for extraction of DNA from different types of fabrics. Bacterial community profiles could be successfully generated with DNA extracted from real life samples, together with identification of different bacterial genera on fabrics. The study opens up venues to study effect of environmental factors on microbial load on fabrics. Also, such a technique will aid correlation between microbial load and types of fabric so as to come up with recommendation for fabrics bearing minimal microbial load for medical and hospitality sectors.
References
Callewaert C, De Maeseneire E, Kerckhof FM, Verliefde A, Van de Wiele T and Boon N 2014 Microbial odor profile of polyester and cotton clothes after a fitness session. Appl. Environ. Microbiol. 80 6611–6619
Dent LL, Marshall DR, Pratap S and Hulette RB 2010 Multidrug resistant Acinetobacter baumannii: a descriptive study in a city hospital. BMC Infect. Dis. 10 196
Faour KD, Shahar N, Ocampo S, Bader T, Zonis Z and Glikman D 2017 Bacteria without borders: A high carriage rate of antibiotic-resistant bacteria among Syrian children hospitalized in Galilee Medical Centre. Harefuah 156 298–301
Han X and Andrade R 2005 Brevundimonas diminuta infections and its resistance to fluoroquinolones. J. Antimicrob. Chemother. 55 853–859
Krieg NR and Holt JG 1984 Bergey’s manual of systematic bacteriology 1st edition, volume 1 (Williams & Wilkins, Baltimore)
McCarthy BJ 2001 Bioluminescent assay of microbial contamination on textile materials. Int. Biodeterior. Biodegrad. 48 79–83
Muyzer G and Smalla K 1998 Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Leeuwenhoek 73 127–141
Noble RC and Overman SB 1994 Pseudomonas stutzeri infection a review of hospital isolates and a review of the literature. Diagn. Microbiol. Infect. Dis. 19 51–56.
Teufel L 2006 Improved methods for the investigation of the interaction between textiles and microorganisms. Lenzinger Berichte 85 54–60
Teufel L, Schuster KC, Merschak P, Bechtold T and Redl B 2008 Development of a fast and reliable method for the assessment of microbial colonization and growth on textiles by DNA quantification. J. Mol. Microbiol. Biotechnol. 14 193–200
Zhang L, Pan Y, Wang K, Zhang X, Zhang S, Fu X, Zhang C and Jiang J 2015 Pseudomonas songnenensis sp. nov., isolated from saline and alkaline soils in Songnen Plain, China. Antonie Leeuwenhoek 107 711–721
Acknowledgements
SV wishes to acknowledge fellowship received from DST-INSPIRE towards her doctoral work. The authors wish to thank IIT Delhi for funding under the Faculty Interdisciplinary Research Project (MI01681G) and Grand Challenge Scheme (MI1798G).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Varshney, S., Sain, A., Anand, G. et al. A robust method for nucleic acid extraction from fabrics to study bacterial diversity. J Biosci 44, 123 (2019). https://doi.org/10.1007/s12038-019-9935-x
Published:
DOI: https://doi.org/10.1007/s12038-019-9935-x