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Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases

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Abstract

Pyruvate carboxylase (Pyc) catalyzes formation of oxaloacetic acid from pyruvic acid by fixing one mole of CO2. Many evidences have confirmed that biosynthesis of some different kinds of organic acids and intracellular and extracellular lipids is driven by Pyc and over-expression of the PYC gene in the industrial microorganisms can promote production of the different kinds of organic acids and intracellular and extracellular lipids. Therefore, the Pyc from different sources is regarded as a key enzyme in microbial biotechnology and is an important target for metabolic engineering of the industrial microbial strains. However, very little is known about the native Pycs and their functions and regulation in the industrial microorganisms.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 31770061).

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    Authors and Affiliations

    1. College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, 266003, People’s Republic of China

      Shou-Feng Zhao, Zhe Chi, Guang-Lei Liu, Long-Fei Wu & Zhen-Ming Chi

    2. Central Laboratories, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, People’s Republic of China

      Shou-Feng Zhao

    3. Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Yushan Road, No. 5, Qingdao, 266003, People’s Republic of China

      Zhe Chi & Guang-Lei Liu

    4. Department of Biology, Shantou University, Shantou, 515063, People’s Republic of China

      Zhong Hu

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    1. Shou-Feng Zhao
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    2. Zhe Chi
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    Correspondence to Zhen-Ming Chi.

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    Communicated by BJ Rao.

    Corresponding editor: BJ Rao

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    Zhao, SF., Chi, Z., Liu, GL. et al. Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases. J Biosci 44, 47 (2019). https://doi.org/10.1007/s12038-019-9853-y

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